PERINEURIUM ORIGINATES FROM FIBROBLASTS - DEMONSTRATION INVITRO WITH A RETROVIRAL MARKER
PERINEURIUM ORIGINATES FROM FIBROBLASTS - DEMONSTRATION INVITRO WITH A RETROVIRAL MARKER
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DOI：
10.1126/science.2492115
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发表时间：
1989-01-13
期刊：
影响因子：
56.9
通讯作者：
SANES, JR
中科院分区：
文献类型：
--
作者：
BUNGE, MB;WOOD, PM;SANES, JR
神经外膜是一种细胞鞘，在周围神经系统的神经纤维束周围形成一种保护性屏障。在一项旨在确定神经膜细胞来源的研究中，使用了一种培养系统，在该系统中，感觉神经元、雪旺细胞和成纤维细胞的纯化群体重组后形成神经膜。重组前，雪旺细胞或成纤维细胞通过感染有缺陷的重组逆转录病毒进行标记，其基因产物β-半乳糖苷酶在感染细胞的后代中可被组织化学检测到。当成纤维细胞被感染时，神经膜细胞被标记，而当雪旺细胞被感染时，则不被标记。因此，神经膜来自体内的成纤维细胞，暗示着体内也是如此。
A cellular sheath, the perineurium, forms a protective barrier around fascicles of nerve fibers throughout the peripheral nervous system. In a study to detrmine the cellular origin of perineurium, a culture system was used in which perineurium forms after purified populations of sensory neurons, Schwann cells, and fibroblasts are recombined. Before recombination, the Schwann cells or the fibroblasts were labeled by infection with a defective recombinant retrovirus whose gene product, .beta.-galactosidase, is histochemically detectable in the progeny of infected cells. Perineurial cells were labeled when fibroblasts had been infected but not when Schwann cells had been infected. Thus, perineurium arises from fibroblasts in vivo and, by implication, in vivo as well.