Pronuclear microinjection.
Pronuclear microinjection.
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DOI：
10.1089/15362300152725936
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发表时间：
2001-01-01
期刊：
影响因子：
--
通讯作者：
Wall, R J
中科院分区：
文献类型：
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作者：
Wall, R J
去年是“转基因”一词发明和原核显微注射发展 20 周年，原核显微注射是一种简单的技术，旨在将几乎所有生物体的遗传信息转移到哺乳动物身上。经过二十年的使用，原核显微注射方案与戈登和拉德尔描述的可靠（如果不是有效）方法相比几乎没有变化。经验告诉我们，一旦显微注射技术完善，只需关注几个参数即可成功生产转基因动物。我们将讨论这些参数，以及一些有望最终提高原核显微注射方法效率的新创新。
Last year marked the 20th anniversary of the invention of the term "transgenic" and the development of pronuclear microinjection, a straightforward technique designed to transfer genetic information from nearly any living organism to mammals. After two decades of use, pronuclear microinjection protocols have changed little from the reliable, if not efficient, method described by Gordon and Ruddle. Experience has taught us that once microinjection skills are perfected there are only a few parameters one needs to be concerned about to successfully produce transgenic animals. Those parameters will be discussed, as will some new innovations that promise to finally increase efficiency of pronuclear microinjection methodology.