Intra- and intercellular fluctuations in Min-protein dynamics decrease with cell length
Intra- and intercellular fluctuations in Min-protein dynamics decrease with cell length
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DOI：
10.1073/pnas.0911708107
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发表时间：
2010-04-06
影响因子：
11.1
通讯作者：
Kruse, Karsten
中科院分区：
文献类型：
--
作者：
Fischer-Friedrich, Elisabeth;Meacci, Giovanni;Kruse, Karsten
蛋白质在空间和时间上的自组织对于细胞过程的功能至关重要。通常，这种组织是在由于所涉及的分子数量较少而存在强烈随机波动的情况下进行的。我们报道了在短小的大肠杆菌细胞中，最小蛋白分布在两个细胞一半之间的随机切换。计算模型提供了强有力的证据，证明宏观开关起源于分子尺度上的微观噪声。在较长的细菌中，这种切换变成了定位分割面所需的规则振荡。随着模式变得更加规则，细胞之间的可变性也减少，这表明最小蛋白活性的调节依赖于细胞长度。
Self-organization of proteins in space and time is of crucial importance for the functioning of cellular processes. Often, this organization takes place in the presence of strong random fluctuations due to the small number of molecules involved. We report on stochastic switching of the Min-protein distributions between the two cell halves in short Escherichia coli cells. A computational model provides strong evidence that the macroscopic switching is rooted in microscopic noise on the molecular scale. In longer bacteria, the switching turns into regular oscillations that are required for positioning of the division plane. As the pattern becomes more regular, cell-to-cell variability also lessens, indicating cell length-dependent regulation of Min-protein activity.