Raman imaging of two orthogonal planes within cortical bone
Raman imaging of two orthogonal planes within cortical bone
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DOI：
10.1016/j.bone.2007.04.200
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发表时间：
2007-09-01
期刊：
影响因子：
4.1
通讯作者：
Fratzl, P.
中科院分区：
文献类型：
--
作者：
Kazanci, M.;Wagner, H. D.;Fratzl, P.
板层骨的强度主要受其矿化胶原纤维的组织和材料组成的影响。在本研究中，拉曼显微光谱和成像分析，研究了正常人股骨中段骨立方体样标本的空间分辨率为1-2 μ m。分析了纵向和横向方向上相同的骨薄层，这使我们能够根据偏振方向分离出取向和成分相关的拉曼线。这种方法提供了有关板层骨取向和骨成分变化的信息。结果表明，nu（1）PO 4与酰胺I的比值主要显示板层骨取向; nu（2）PO 4与酰胺III和CO 3与nu（2）PO 4的比值显示骨组成的变化。nu（2）PO 4与酰胺III的比值在间质骨区域较高，而CO 3与nu（2）PO 4的比值在同一区域较低。本研究提供了新的见解，从两个正交方向的板层骨组织的组织。(C)2007年爱思唯尔公司All rights reserved.
The lamellar bone's strength is mainly affected by the organization of its mineralized collagen fibers and material composition. In the present study, Raman microspectroscopic and imaging analyses were employed to study a normal human femoral midshaft bone cube-like specimen with a spatial resolution of similar to 1-2 mu m. Identical bone lamellae in both longitudinal and transverse directions were analyzed, which allowed us to separate out orientation and composition dependent Raman lines, depending on the polarization directions. This approach gives information about lamellar bone orientation and variation in bone composition. It is shown that the nu(1) PO4 to amide I ratio mainly displays lamellar bone orientation; and nu(2) PO4 to amide III and CO3 to nu(2) PO4 ratios display variation in bone composition. The nu(2) PO4 to amide III ratio is higher in the interstitial bone region, whereas the CO3 to nu(2) PO4 ratio has lower values in the same region. The present study provides fresh insights into the organization of a lamellar bone tissue from two orthogonal orientations. (C) 2007 Elsevier Inc. All rights reserved.