Dandruff as a potential source of DNA in forensic casework.
Dandruff as a potential source of DNA in forensic casework.
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DOI：
10.1520/jfs14326j
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发表时间：
1998-07
影响因子：
1.6
通讯作者：
M. Lorente;C. Entrala;J. Lorente;J. C. Alvarez;E. Villanueva;B. Budowle
中科院分区：
文献类型：
--
作者：
M. Lorente;C. Entrala;J. Lorente;J. C. Alvarez;E. Villanueva;B. Budowle
头皮屑是一种皮肤的临床病变，从组织学上看，它由正角化团块组成，在脂溢性皮炎和银屑病等炎症性疾病中可见微小的不全角化病灶。因此，在头皮屑中应该能发现一些有核细胞，也就有可能从头皮屑中提取出可用于法医鉴定的DNA。由于我们参与的一个特殊案例，我们进行了一项研究，以确定是否能从头皮屑中提取DNA，以及两种最广泛使用的提取技术（Chelex法和有机法）是否适用。结果表明，从仅1.0到1.5毫克的头皮屑中就可以获得足够量的DNA（超过30到40纳克）。两种方法都能提取出DNA，不过有机法似乎提取得更多（Chelex法为72.5纳克，有机法为183.3纳克）。所有提取的DNA样本在HUMTH01和HUMvWA基因座上都能正确分型。因此，头皮屑可被视为法医鉴定中DNA的一个潜在来源。
Dandruff is a clinical alteration of the skin that consists histologically of orthokeratotic clumps with minute parakeratotic foci found in inflammatory pathologies such as seborrheic dermatitis and psoriasis. Therefore, some nucleated cells should be found in dandruff and hence there is a possibility that forensically typeable DNA could be extracted from dandruff. Because of a particular case in which we were involved, a study was carried out to determine whether or not DNA could be extracted from dandruff, and if the two most widely used extraction techniques (Chelex and organic) would be applicable. Results show that sufficient quantities of DNA (more than 30 to 40 ng) can be obtained from as little as 1.0 to 1.5 mg of dandruff. Both methods yield DNA, although the organic procedure seems to yield more (72.5 ng Chelex vs. 183.3 ng organic). All the DNA samples extracted were typed correctly for the loci HUMTH01 and HUMvWA. Therefore, dandruff can be considered a potential source of DNA for forensic identification.