Mass spectrometric analysis of carisoprodol and meprobamate in rat brain microdialysates.
Mass spectrometric analysis of carisoprodol and meprobamate in rat brain microdialysates.
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大鼠脑微透析液中卡立普多和甲丙氨酯的质谱分析。
DOI：
10.1002/jms.3799
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发表时间：
2016
期刊：
影响因子：
--
通讯作者：
Forster,MichaelJ
中科院分区：
文献类型：
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作者：
Prokai,Laszlo;Fryčák,Petr;Nguyen,Vien;Forster,MichaelJ
我们报道了几种基于质谱的方法，用于测定从大鼠脑内微透析获得的样品中的异丙醇和甲丙酸。在那些希望在没有色谱分离的情况下进行分析从而提高通量的技术中，基于芯片的纳米电喷雾电离和使用常压固体分析探针分别由于检测灵敏度和硬电离不足而未能达到要求。虽然实时直接分析提供了所需的软电离，但串联质谱分析的缺点还包括检测灵敏度不足，此外，定量重现性差。为此，建立了液相色谱联用常压化学电离串联质谱法测定人工脑脊液中异丙醇和甲丙酸酯的方法。样品不需要脱盐和/或提取。该实验通过颅内微透析在体内取样，提供了大鼠全身给药卡异丙醇后大脑伏隔核区域药物及其主要代谢物的时间分辨浓度谱。版权所有©2016 John Wiley & Sons， Ltd。
We report the evaluation of several mass spectrometry‐based methods for the determination of carisoprodol and meprobamate in samples obtained from the rat brain byin vivointracranial microdialyis. Among the techniques that aspire to perform analyses without chromatographic separation and thereby increase throughput, chip‐based nanoelectrospray ionization and the use of an atmospheric pressure solids analysis probe fell short of requirements because of insufficient detection sensitivity and hard ionization, respectively. Although direct analysis in real time provided the required soft ionization, shortcomings of a tandem mass spectrometry‐based assay also included inadequate detection sensitivity and, in addition, poor quantitative reproducibility. Therefore, liquid chromatography coupled with atmospheric pressure chemical ionization tandem mass spectrometry was developed to determine carisoprodol and meprobamate from artificial cerebrospinal fluid as the medium. No desalting and/or extraction of the samples was necessary. The assay, combined within vivosampling via intracranial microdialyis, afforded time‐resolved concentration profiles for the drug and its major metabolite from the nucleus accumbens region of the brain in rats after systemic administration of carisoprodol. Copyright © 2016 John Wiley & Sons, Ltd.