Cre/loxP-mediated chromosome engineering of the mouse genome.
Cre/loxP-mediated chromosome engineering of the mouse genome.
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DOI：
10.1007/978-3-540-35109-2_2
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发表时间：
2007
影响因子：
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通讯作者：
V. Brault;Vanessa Besson;L. Magnol;A. Duchon;Y. Hérault
中科院分区：
文献类型：
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作者：
V. Brault;Vanessa Besson;L. Magnol;A. Duchon;Y. Hérault
与许多其他基因组修改一起，染色体工程提供了一个非常强大的工具来加速哺乳动物基因组的功能分析。这项基于Cre/loxP系统的技术越来越多地在科学界使用，以便在目标感兴趣的区域产生携带缺失、重复、倒置和易位的新染色体。在这篇综述中，我们将介绍这项技术的基本原理，无论是在体内还是在体外，我们将简要描述一些应用，以提供非常有价值的遗传工具，破译哺乳动物基因组组织和在小鼠身上分析人类疾病。
Together with numerous other genome modifications, chromosome engineering offers a very powerful tool to accelerate the functional analysis of the mammalian genome. The technology, based on the Cre/loxPsystem, is used more and more in the scientific community in order to generate new chromosomes carrying deletions, duplications, inversions and translocations in targeted regions of interest. In this review, we will present the basic principle of the technique either in vivo or in vitro and we will briefly describe some applications to provide highly valuable genetic tools, to decipher the mammalian genome organisation and to analyze human diseases in the mouse.