Analysis of plasma proteome from cases of the different traditional Chinese medicine syndromes in patients with chronic hepatitis B
Analysis of plasma proteome from cases of the different traditional Chinese medicine syndromes in patients with chronic hepatitis B
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DOI：
10.1016/j.jpba.2011.10.002
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发表时间：
2012-02-05
影响因子：
3.4
通讯作者：
Wei, Mei
中科院分区：
文献类型：
--
作者：
Liu, Youping;Liu, Peng;Wei, Mei
采用二维凝胶电泳 (2-DE) 和基质辅助激光解吸/电离飞行时间质谱 (MALDI-TOF-MS) 蛋白质组分析方法探讨慢性乙型肝炎 (CHB) 患者血浆蛋白质组与不同中医证候之间的联系。与健康供体血浆蛋白质组比较，发现慢性乙型肝炎湿热瘀阻中焦证、肝郁脾虚证、脾肾阳虚证、肝肾阴虚证、血瘀络络证等五种证型的蛋白表达变化（P < 0.05）。在慢性乙型肝炎的五种中医证型中，免疫球蛋白J链（IGJ）和C反应蛋白（CRP）表达上调，而触珠蛋白（HPT）、视网膜结合蛋白（RBP）和玻连蛋白表达下调。为了进一步证实这些结果，通过 ELISA 对更多血浆样本中的四种蛋白质（包括 CRP、IGJ、HPT 和 RBP）进行了定量。结果表明，蛋白质水平的变化与2-DE实验一致。重要的是，血浆中IGJ水平的上调趋势与CHB的不同中医证型相关（P < 0.05）。我们的研究结果表明，IGJ 可能作为诊断慢性乙型肝炎患者不同中医证候的新型潜在生物标志物。 (C) 2011 Elsevier B.V. 保留所有权利。
A proteomic analysis method, two dimensional gel electrophoresis (2-DE) followed by matrix-assisted laser desorption/ionization time-of-flight MS (MALDI-TOF-MS), was used to explore the link between plasma proteome and the different syndromes of traditional Chinese medicine (TCM) in patients with chronic hepatitis B (CHB). In compared with the plasma proteomes from health donors, the alterations in protein expression from cases of the five TCM syndromes, including damp heat stasis in the middle-Jiao syndrome, liver Qi stagnation and spleen deficiency syndrome, spleen and kidney Yang deficiency syndrome, liver and kidney Yin deficiency syndrome, and blood stasis into collateral syndrome with CHB were identified (P < 0.05). In the cases of the five TCM syndromes with CHB, immunoglobulin J-chains (IGJ) and C-reactive protein (CRP) were up-regulated, while haptoglobin (HPT), retinal binding protein (RBP) and vitronectin were down-regulated. To further confirm these results, four proteins, including CRP, IGJ, HPT and RBP, from more plasma samples were quantified by ELISA. The results showed that the changes of protein levels were consistent with those from the 2-DE experiment. Importantly, the upregulation tendency of IGJ level in plasma is related with the differentTCM syndromes with CHB (P < 0.05). Our results show that IGJ may serve as a novel potential biomarker for diagnosis of the different TCM syndromes in patients with CHB. (C) 2011 Elsevier B.V. All rights reserved.