Trimodal spectroscopy for the detection and characterization of cervical precancers in vivo
Trimodal spectroscopy for the detection and characterization of cervical precancers in vivo
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DOI：
10.1067/mob.2002.121075
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发表时间：
2002-03-01
影响因子：
9.8
通讯作者：
Feld, MS
中科院分区：
文献类型：
--
作者：
Georgakoudi, I;Sheets, EE;Feld, MS
目的：本研究的目的是评估潜在的3个光谱技术（内源性荧光，漫反射，光散射）单独和组合（三峰光谱）检测宫颈鳞状上皮内lessones.Study设计：这项研究进行了44例谁接受阴道镜检查的异常巴氏涂片的评价。从阴道镜检查正常和异常部位收集荧光和反射光谱，并进行分析，以提取有关组织生物化学和形态学状况的定量信息。这一信息进行了比较，与组织病理学分类，诊断算法的开发和验证与使用logistic回归和cross-validation.Results：诊断显着差异存在于荧光生化成分，散射特性，和上皮细胞核形态的宫颈鳞状上皮内病变和非鳞状上皮内病变。三峰光谱是一个上级工具，用于检测宫颈鳞状上皮内病变比任何1的techniques alone.CONCLUSION：三峰光谱有可能提高在体内检测宫颈癌前病变。
OBJECTIVE: The objective of this study was to assess the potential of 3 spectroscopic techniques (intrinsic fluorescence, diffuse reflectance, and light scattering) individually and in combination (trimodal spectroscopy) for the detection of cervical squamous intraepithelial lesions.STUDY DESIGN: The study was conducted with 44 patients who underwent colposcopy for the evaluation of an abnormal Papanicolaou smear. Fluorescence and reflectance spectra were collected from colposcopically normal and abnormal sites and analyzed to extract quantitative information about tissue biochemistry and morphologic condition. This information was compared with histopathologic classification, and diagnostic algorithms were developed and validated with the use of logistic regression and cross-validation.RESULTS: Diagnostically significant differences exist in the composition of fluorescing biochemicals, the scattering properties, and the epithelial cell nuclear morphology of cervical squamous intraepithelial lesions and non-squamous intraepithelial lesions. Trimodal spectroscopy is a superior tool for the detection of cervical squamous intraepithelial lesions than any 1 of the techniques alone.CONCLUSION: Trimodal spectroscopy has the potential to improve the in vivo detection of precancerous cervical changes.