RAPID MULTIPLE MASS CONSTITUENT ANALYSIS OF BIOLOGICAL CELLS
RAPID MULTIPLE MASS CONSTITUENT ANALYSIS OF BIOLOGICAL CELLS
复制标题
生物细胞的快速多种质量成分分析
DOI：
10.1111/j.1749-6632.1969.tb12668.x
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发表时间：
1969
影响因子：
5.2
通讯作者：
M. Melamed
中科院分区：
文献类型：
--
作者：
L. Kamentsky;M. Melamed
An instrument, which we shall call the rapid cell spectrophotometer, has been described' for measuring and displaying certain optical properties of biological cells flowing in liquid suspension at rates up to 1,000 cells per second. This instrument was designed to characterize each cell of populations of the order of 100,000 cells or more in a few minutes so that a few unusual cells could be identified. In a later paper2 we described extensions of the rapid cell spectrophotometer whereby the cells may be physically separated according to functions of up to four simultaneous optical measurements on each cell. We have also shown why scanning and pattern recognition techniques were abandoned in favor of the use of physical measurements on the c e k 3 The examination of 1,000 cells per second led to scanning and processing rates of los to lo7 bits per second even using video data reduction methods, such as spatial differentiation, developed during the last few year^.^,^ We felt that the current scanning techniques will not yield sufficient signal-to-noise ratios at this speed and that current decision netw o r k ~ ~ , ~ are not sufficiently developed to manipulate the data rapidly enough. Accurate estimates of mass constituents of cells are often needed and scanning devices* or techniques such as the two wavelength methodgJO have been developed for this purpose. The three major problems in obtaining quantitative estimates of mass constituents due to light scatter, multiple constituent absorptions, and varying constituent distributions are analyzed. We will describe techniques for reducing these errors by modifications of the instrument and techniques, and by the use of a computer to perform mass-constituent calculations as each sample is run. The computer will also provide for a more quantitative display of data and more sophisticated decision procedures for cell sorting. The rapid cell spectrophotometer is presently being interfaced with an IBM 1130 computer to perform these functions and this paper is the basis for some of the programs now being developed for the computer.
