Mapping of QTLs for physicochemical properties in Japonica rice
Mapping of QTLs for physicochemical properties in Japonica rice
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DOI：
10.1270/jsbbs.56.253
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发表时间：
2006-09-01
期刊：
影响因子：
2.4
通讯作者：
Matsue, Yuji
中科院分区：
文献类型：
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作者：
Wada, Takuya;Uchimura, Yosuke;Matsue, Yuji
为了建立一个分子标记辅助选择（MAS）体系，以选育食味品质优良的水稻新品种，利用由92个粳稻品种（食味品质差的品种盛泽和食味品质好的品种越光）杂交而成的重组自交系（RI）群体，对稻米理化性状进行QTL分析。利用119个简单重复序列（SSR）标记构建了一张连锁图谱。通过测定稻米的蛋白质含量、直链淀粉含量和质构特性，对两年的稻米理化特性进行了评价。蛋白质含量的QTL分析显示，在第2、6和9染色体上有3个QTL。在第3、7、9和12染色体上检测到4个控制直链淀粉含量的QTL。在第3染色体上定位到一个控制质地性状的QTL。与这些QTL连锁的DNA标记将被用于优良食味品质的MAS。
In order to establish a system for marker assisted selection (MAS) to develop new rice cultivars with good eating quality, we conducted QTL analyses for physicochemical properties of rice by using a recombinant inbred (RI) population consisting of 92 lines derived from a cross between closely related japonica cultivars, Moritawase (a cutivar with low eating quality) and Koshihikari (a cultivar with good eating quality). We developed a linkage map with 119 simple sequence repeat (SSR) markers. Physicochemical properties of rice were evaluated two years by measuring protein content, amylose content and textural characteristics. QTL analysis for protein content revealed three QTLs on chromosome 2, 6 and 9. Four QTLs for amylose content were detected on chromosome 3, 7, 9 and 12. One QTL for texture characteristics were identified on chromosome 3. The DNA markers linked to these QTLs will be used for the MAS for good eating quality.