Crystallization of lysozyme with (R)-, (S)- and (RS)-2-methyl-2,4-pentanediol.
Crystallization of lysozyme with (R)-, (S)- and (RS)-2-methyl-2,4-pentanediol.
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溶菌酶与（R） - ，（S） - 和（RS）-2-甲基-2,4-五苯二醇的结晶。
DOI：
10.1107/s1399004714025061
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发表时间：
2015-03
期刊：
影响因子：
--
通讯作者：
Asherie N
中科院分区：
文献类型：
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作者：
Stauber M;Jakoncic J;Berger J;Karp JM;Axelbaum A;Sastow D;Buldyrev SV;Hrnjez BJ;Asherie N
溶菌酶与(R)-2-甲基-2，4-戊二醇结晶比与(S)-2-甲基-2，4-戊二醇结晶得到更有序的晶体和更高分辨率的蛋白质结构。结果表明，与手性添加剂的手性相互作用在蛋白质晶体的形成中是重要的。手性控制结晶在小分子领域已有大量先例，但对手性在蛋白质结晶中的作用知之甚少。在这项研究中，溶菌酶在手性添加剂2-甲基-2，4-戊二醇(MPD)的存在下，分别使用R和S对映体以及外消旋RS混合物进行结晶。用(R)-MPD生长的晶体具有最高的有序性和最高分辨率的蛋白质结构。这一结果与在(R)-MPD和(RS)-MPD生长的晶体中以(R)-MPD形成晶体接触的观察结果一致，表明溶菌酶与该对映体之间存在优先相互作用。这些发现表明，手性相互作用在蛋白质结晶中是重要的。
Crystallization of lysozyme with (R)-2-methyl-2,4-pentanediol produces more ordered crystals and a higher resolution protein structure than crystallization with (S)-2-methyl-2,4-pentanediol. The results suggest that chiral interactions with chiral additives are important in protein crystal formation. Chiral control of crystallization has ample precedent in the small-molecule world, but relatively little is known about the role of chirality in protein crystallization. In this study, lysozyme was crystallized in the presence of the chiral additive 2-methyl-2,4-pentanediol (MPD) separately using the R and S enantiomers as well as with a racemic RS mixture. Crystals grown with (R)-MPD had the most order and produced the highest resolution protein structures. This result is consistent with the observation that in the crystals grown with (R)-MPD and (RS)-MPD the crystal contacts are made by (R)-MPD, demonstrating that there is preferential interaction between lysozyme and this enantiomer. These findings suggest that chiral interactions are important in protein crystallization.