Immunogenicity of Haemophilus influenzae type b polysaccharide--diphtheria toxoid conjugate vaccine in adults.
Immunogenicity of Haemophilus influenzae type b polysaccharide--diphtheria toxoid conjugate vaccine in adults.
复制标题
B型流感嗜血杆菌多糖-白喉类毒素结合疫苗的成人免疫原性。
DOI：
10.1016/s0022-3476(84)80350-9
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发表时间：
1984
期刊：
影响因子：
--
通讯作者：
MunsonJr,RS
中科院分区：
文献类型：
--
作者：
Granoff,DM;Boies,EG;MunsonJr,RS
b型流感嗜血杆菌的荚膜多糖对人类婴儿来说是一种较差的免疫原。为了增强免疫原性，这种多糖与白喉类毒素共价偶联，并在成年志愿者中测试了缀合物作为疫苗的效果。注射两次PRP-D疫苗，间隔一个月。该组中的抗 PRP 抗体反应与接受相当剂量（20 μg）常规 PRP 疫苗的组中的抗 PRP 抗体反应进行了比较。两种疫苗的耐受性均良好。单次注射 PRP-D 的免疫原性明显高于 PRP，1 个月后可引发更高的总抗 PRP 抗体血清浓度（地理平均值分别为 248 和 62 μg/ml；P<0.001）。此外，PRP-D 组中观察到较高浓度的 IgG 抗 PRP 抗体（P<0.001）。重新注射疫苗1个月后，接受PRP-D的受试者的总抗体出现小幅但显着的下降（P=0.03），而接受PRP组的血清抗体浓度则保持不变。 12个月时，两组的抗体浓度没有显着差异。在每个疫苗组中三个最高反应者和三个最低反应者的混合血清中测试了杀菌活性和被动保护活性（幼年大鼠模型）； PRP 和 PRP-D 疫苗均诱导具有生物活性的抗 PRP 抗体。因此，发现 PRP-D 可以引发具有生物活性的血清抗体，并且在成人中比 PRP 疫苗更具免疫原性；然而，较高浓度抗体的持续时间是短暂的。
The capsular polysaccharide ofHaemophilus influenzaetype b is a poor immunogen in human infants. In an attempt to enhance immunogenicity, this polysaccharide was covalently coupled to diphtheria toxoid and the conjugate tested as a vaccine in adult volunteers. Two injections of PRP-D vaccine were given, separated by one month. The anti-PRP antibody responses in this group were compared with those in a group receiving a comparable dose (20 μg) of conventional PRP vaccine. Both vaccines were well tolerated. A single injection of PRP-D was significantly more immunogenic than PRP, eliciting higher serum concentrations of total anti-PRP antibody 1 month later (geo means of 248 and 62 μg/ml, respectively;P<0.001). In addition, higher concentrations of IgG anti-PRP antibody were observed in the PRP-D group (P<0.001). One month after reinjection of vaccine, subjects receiving PRP-D showed a small but significant decline in total antibody (P=0.03), whereas the serum antibody concentrations in the group that received PRP remained unchanged. At 12 months, the antibody concentrations of the two groups were not significantly different. Bactericidal activity and passive protection activity (infant rat model) were tested in pooled sera from the three highest and three lowest responders in each vaccine group; both PRP and PRP-D vacines induced biologically active anti-PRP antibody. Thus PRP-D was found to elicit biologically active serum antibody and to be more immunogenic in adults than PRP vaccine; however, the duration of higher concentrations of antibody was transient.