EXPRESSION OF SURFACE-ANTIGENS DISTINGUISHING NAIVE AND PREVIOUSLY ACTIVATED LYMPHOCYTES IN BRONCHOALVEOLAR LAVAGE FLUID
EXPRESSION OF SURFACE-ANTIGENS DISTINGUISHING NAIVE AND PREVIOUSLY ACTIVATED LYMPHOCYTES IN BRONCHOALVEOLAR LAVAGE FLUID
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DOI：
10.1136/thx.45.5.391
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发表时间：
1990-05-01
期刊：
影响因子：
10
通讯作者：
HANCE, AJ
中科院分区：
文献类型：
--
作者：
DOMINIQUE, S;BOUCHONNET, F;HANCE, AJ
动物研究表明，吸入抗原对未引发的（“初始”）T 淋巴细胞的最初激活可能发生在肺外，随后又被招募到肺中。如果这是真的，肺中存在的所有淋巴细胞都应该显示出先前激活的证据。为了检验肺泡表面淋巴细胞的这一假设，对从正常受试者和结节病患者的血液和支气管肺泡灌洗液中回收的 T 淋巴细胞测量了表面抗原的表达，这些抗原可区分未引发的细胞和先前激活的细胞（CD45RS、CD29、Leu-8）。正常受试者和结节病患者的灌洗液中很少有 T 淋巴细胞表达“原始”细胞所预期的 Leu 8+ 或 CD45RAbright 表型；更多的细胞具有“原始”细胞所预期的 CD29dull 表型，尽管八名受试者中的五名具有低于 2% 的此类细胞。这些发现支持这样的结论：呼吸道表面唯一存在的 T 淋巴细胞是识别个体之前遇到过的抗原的 T 淋巴细胞。需要进一步的研究来确定“原始”T 淋巴细胞是否存在于其他肺室中。
Studies in animals suggest that the initial activation of unprimed ("naive") T lymphocytes by inhaled antigens may occur outside the lung with later recruitment to the lung. If this is true all lymhocytes persent in the lung should show evidence of prior activation. To test this hypothesis for lymphocytes present on the alveolar surface, the expression of surface antigens, which distinguish unprimed from previously activated cells (CD45RS, CD29, Leu-8), were measured on T lymphocytes recovered from blood and bronchoalveolar lavage fluid from normal subjects and patients with sarcoidosis. Few T lymphocytes from the lavage fluid of normal subjects and patients with sarcoidosis expressed the Leu 8+ or CD45RAbright phenotype expected for "naive" cells; more cells had the CD29dull phenotype expected for "naive" cells, though five of eight subjects had under 2% of such cells. These findings support the conclusion that the only T lymphocytes present on the surface of the respiratory tract are those recognising antigens that have been previously encountered by the individual. Further studies are required to determine whether "naive" T lymphocytes are present in other lung compartments.