Birth outcomes and IGF2 methylation in Tibetan and Han Mother-Newborn Pairs in hypobaric hypoxia high-altitude area.
Birth outcomes and IGF2 methylation in Tibetan and Han Mother-Newborn Pairs in hypobaric hypoxia high-altitude area.
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低压缺氧高海拔地区藏族和汉族母婴对的出生结局及IGF2甲基化
DOI：
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发表时间：
2023
期刊：
影响因子：
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通讯作者：
Wen Peng
中科院分区：
文献类型：
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作者：
Wenxiu Jian;Pin Sun;Xuejun Wang;Pinhua Wang;Jiangzuo Zhaxi;Yangrui Zhang;Shulin Wang;Dexiong Zhao;Yuemei Fan;Liehong Wang;Wen Peng
背景资料：胰岛素样生长因子2（IGF 2）甲基化在P3启动子区和出生之间的关系。在低压缺氧环境中的结果从来没有被调查。本研究通过种族和海拔来检验和比较出生结果和IGF 2甲基化的相关性。方法：采用出生队列研究方法，对406对出生在青藏高原的母亲和新生儿进行研究。数据收集通过访谈使用结构问卷或从医疗记录中提取。对母亲外周血和脐带血中P3启动子区域的IGF 2甲基化进行焦磷酸测序。比较高海拔地区汉族（HHA，n=164，2000- 3500 m）、高海拔地区藏族（THA，n=42，2000- 3500 m）和超高海拔地区藏族（TUHA，n=200，3500 m及以上）3组的出生结局和IGF 2甲基化水平。结果：TUHA组巨大儿发生率（7.5%）高于THA组（0.0%）HHA组（2.4%）和HHA组（2.4%）的母血IGF 2甲基化水平低于THA组（P=0.008）。在THA和HHA之间没有发现IGF 2甲基化水平的差异。母亲外周血中IGF 2甲基化水平与巨大儿的风险降低相关（RR= 0.726，95%CL [0.528，0.998]，P=0.049）。二、结论：升高的海拔似乎与母亲P3启动子区域IGF 2甲基化水平降低相关，并且该区域的母亲IGF 2甲基化水平与低压缺氧西藏高原环境中新生儿巨大儿风险降低相关。
Background: The relationship between Insulin-like growth factor 2 (IGF2) methylation in the P3 promoter region and birth .outcomes in a hypobaric-hypoxia environment has never been investigated. This study examined the association and .compared birth outcomes and IGF2 methylation in this region by ethnicity and altitude. .Methods: Four hundred and six (406) mother and newborn pairs in the Tibetan Plateau were enrolled in a birth cohort .study. Data were collected through interviews using structural questionnaires or extracted from medical records. .Pyrosequencing was performed for IGF2 methylation in the P3 promoter region in maternal peripheral and umbilical cord .blood. Birth outcomes and IGF2 methylation were compared among three groups: Han in high altitude (HHA, n=164, 2000-.3500m), Tibetan in high altitude (THA, n=42, 2000-3500m), and Tibetan in ultra-high altitude (TUHA, n=200, 3500m and .higher)..Results: TUHA seemed to have a higher prevalence of macrosomia (7.5%) than both THA (0.0.%) and HHA (2.4%) and a .lower IGF2 methylation level in maternal blood than THA (P=0.008). No difference in the IGF2 methylation levels was found .between THA and HHA. The IGF2 methylation levels in maternal peripheral blood were associated with a reduced risk of .macrosomia (RR= 0.726, 95% CL [0.528,0.998], P=0.049) among all mother and newborn pairs. .Conclusions: Increased altitude appears to be associated with decreased maternal IGF2 methylation levels in the P3 .promoter region, and maternal IGF2 methylation levels in this region was associated with reduced risk of macrosomia in.newborns in the hypobaric hypoxic Tibetan Plateau environment.