Improved mesenchymal stem cell seeding on RGD-modified poly(L-lactic acid) scaffolds using flow perfusion
Improved mesenchymal stem cell seeding on RGD-modified poly(L-lactic acid) scaffolds using flow perfusion
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DOI：
10.1002/mabi.200600280
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发表时间：
2007-05-10
影响因子：
4.6
通讯作者：
Sikavitsas, Vassilios I.
中科院分区：
文献类型：
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作者：
Alvarez-Barreto, Jose F.;Sikavitsas, Vassilios I.
精氨酸-甘氨酸-天冬氨酸（Arg-Gly-Asp，RGD）已被广泛用于增加细胞与组织工程三维支架的粘附。然而，在这些支架上的细胞接种仅静态进行，这产生低的细胞接种效率。我们的特点是，第一次，大鼠骨髓间充质干细胞的RGD修饰的聚（L-乳酸）（PLLA）泡沫使用振荡流灌注的种子。在PLLA泡沫上掺入RGD在振荡流灌注接种下以剂量依赖性方式改善支架细胞性。与静态接种相比，振荡流灌注是最有效的接种技术。细胞分离研究表明，细胞粘附是依赖于所施加的流速，并在较高水平的RGD修饰的细胞附着得到加强。
Arg-Gly-Asp (RGD) has been widely utilized to increase cell adhesion to three-dimensional scaffolds for tissue engineering. However, cell seeding on these scaffolds has only been carried out statically, which yields low cell seeding efficiencies. We have characterized, for the first time, the seeding of rat mesenchymal stem cells on RGD-modified poly(L-lactic acid) (PLLA) foams using oscillatory flow perfusion. The incorporation of RGD on the PLLA foams improves scaffold cellularity in a dose-dependent manner under oscillatory flow perfusion seeding. When compared to static seeding oscillatory flow perfusion is the most efficient seeding technique. Cell detachment studies show that cell adhension is dependent on the applied flow rate, and that cell attachment is strengthened at higher levels of RGD modification.