Real-time confocal two-photon fluorescence microscope using a rotating microlens array

Real-time confocal two-photon fluorescence microscope using a rotating microlens array
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使用旋转微透镜阵列的实时共焦双光子荧光显微镜

DOI:
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发表时间:
1999
期刊:
Other Conferences
影响因子:
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通讯作者:
S. Kawata
S. Kawata
中科院分区:
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文献类型:
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作者:
K. Fujita;T. Kaneko;O. Nakamura;M. Oyamada;T. Takamatsu;S. Kawata

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提出在具有微透镜阵列的实时双光子荧光显微镜中使用共焦针孔阵列来提高三维分辨率和深度穿透性能。在我们的实验中观察到所提出的显微镜的横向和轴向分辨率的增强。显示了使用和不使用针孔阵列拍摄的图像的比较,以验证清晰的深度成像性能。
The use of a confocal pinhole-array in a real-time two-photon fluorescence microscope with a microlens array is proposed to increase the three-dimensional resolution and the depth- penetration property. Enhancement of the lateral and axial resolution in the proposed microscope is observed in our experiments. Comparisons of images taken with and without the pinhole-array to verify the sharp depth-imaging performance are shown.
DOI: 10.1126/science.2321027
发表时间: 1990-04-06
期刊: SCIENCE
影响因子: 56.9
作者:
DENK, W;STRICKLER, JH;WEBB, WW
通讯作者: WEBB, WW