Hypercomplementemia in adult patients with IgA nephropathy
Hypercomplementemia in adult patients with IgA nephropathy
复制标题
DOI：
10.1002/jcla.20154
复制
发表时间：
2007-01-01
影响因子：
2.7
通讯作者：
Tomino, Yasuhiko
中科院分区：
文献类型：
--
作者：
Onda, Kisara;Ohi, Hiroyuki;Tomino, Yasuhiko
IgA肾病（IgAN）是慢性肾小球肾炎最常见的形式。虽然补体成分的肾小球沉积是众所周知的，IgAN中血清学补体激活的证据是不确定的。我们推测IgAN患者血清补体成分和调节蛋白水平与其发病机制相关。在本研究中，我们测定了50例IgAN患者和50名健康志愿者的补体成分。采用单向免疫扩散法测定C5、C1抑制物、因子B、C4结合蛋白、因子H和因子I。酶联免疫吸附试验（ELISA）测定甘露糖结合凝集素（MBL）和备解素（P）。患者血清中补体与IgAN临床分级（即，预后良好组、预后较好组、预后较差组、预后不良组）。IgA肾病患者的CH 50、C4、因子B、P、因子1和因子H均显著高于健康对照组。IgA肾病患者血清中C5与C4结合蛋白、C3与C5、C4与B因子之间存在显著相关性。在预后不良组中，C4结合蛋白明显高于其他各组IgAN患者。高补体血症发生在IgAN中，并与补体调节蛋白（CRP）的增加有关。C4结合蛋白分析可用于预测疾病预后。
IgA nephropathy (IgAN) is the most common form of chronic glomerulonephritis. Although glomerular deposition of complement components is well known, the evidence of serological complement activation in IgAN is inconclusive. We hypothesized that serum levels of complement components and regulatory proteins in patients with IgAN are correlated with its pathogenesis. In the present study we measured complement components in 50 patients with IgAN and 50 healthy volunteers. C5, C1 inhibitor, factor B, C4 binding protein, factor H, and factor I were measured with the use of single radial immunodiffusion. Mannose-binding lectin (MBL) and properdin (P) were measured by enzyme-linked immunosorbent assay (ELISA). The correlations among complements in the sera of patients with clinical gradings for IgAN (i.e., the good prognosis group, relatively good prognosis group, relatively poor prognosis group, and poor prognosis group) were evaluated. CH50, C4, factor B, P, factor 1, and factor H were significantly higher in IgAN patients than in healthy controls. There were significant correlations between C5 and C4 binding protein, between C3 and C5, or between C4 and factor B in patients with IgAN. In the poor prognosis group, C4 binding protein was significantly higher than in the other groups of IgAN patients. hypercomplementemia occurs in IgAN and is associated with an increase in complement regulatory protein (CRP). C4 binding protein analyses can be used to predict disease prognosis.