Bordetella pertussis vaccine strains and circulating isolates in Serbia
Bordetella pertussis vaccine strains and circulating isolates in Serbia
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DOI：
10.1016/j.vaccine.2009.11.036
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发表时间：
2010-02-03
期刊：
影响因子：
5.5
通讯作者：
He, Qiushui
中科院分区：
文献类型：
--
作者：
Dakic, Gordana;Kallonen, Teemu;He, Qiushui
在塞尔维亚，全细胞百日咳疫苗于1957年引入。目前的疫苗成分自1985年以来一直在使用，含有1957年至1984年分离的四种百日咳博德特氏菌本土菌株。为了监测细菌种群的变化，对1953年至2000年收集的70株分离株与疫苗株进行了研究。方法包括菌毛血清学分型（Fim）、百日咳毒素基因分型（prn）和百日咳毒素S1亚单位（ptxA）。和脉冲场凝胶电泳分析。自20世纪60年代后期以来，在分离物中观察到从ptxA2到ptxA1的转变。从1980年到1984年的所有分离株都携带ptxA1。1985年，在疫苗中添加了两种携带ptxA1的菌株后，ptxA2等位基因再次出现。等位基因prn1是塞尔维亚分离株中占主导地位，虽然prn3和prn11已检测到自1981年和1984年。prn2等位基因仅在2000年分离的两个菌株中发现。1980年以前血清型Fim2.3消失，此后血清型Fim2占优势。塞尔维亚疫苗株在ptxA和pm方面存在差异。本研究的结果表明，B。塞尔维亚的百日咳感染人群与其他接种疫苗的人群不同，这种差异可能与所使用的疫苗有关。(C)2009爱思唯尔有限公司保留所有权利。
In Serbia, whole cell pertussis vaccine was introduced in 1957. Current composition of the vaccine has been used since 1985 and contains four autochthonous strains of Bordetella pertussis isolated from 1957 to 1984. To monitor changes in bacterial population, 70 isolates collected from 1953 to 2000 were studied together with the vaccine strains. The methods included serotyping of fimbriae (Fim), genotyping of pertactin (prn) and pertussis toxin S1 subunit (ptxA). and pulsed-field gel electrophoresis analysis. Shift from ptxA2 to ptxA1 has been observed in isolates since the late of 1960s. All isolates from 1980 to 1984 harbored ptxA1. Re-appearance of the ptxA2 allele followed an addition of the two strains harboring ptxA1 in the vaccine in 1985. The allele prn1 was predominant among the Serbian isolates, though prn3 and prn11 have been detected since 1981 and 1984. The allele prn2 was found only in two strains isolated in 2000. Serotype Fim2.3 disappeared before 1980 and serotype Fim2 became predominant since then. The Serbian vaccine strains showed differences in ptxA and pm. The results of this present study indicate that the B. pertussis population in Serbia is different from other vaccinated populations and that this difference may be related to the vaccine used. (C) 2009 Elsevier Ltd. All rights reserved.