Isolation and characterization of staphylococci from external auditory meatus of dogs with or without otitis externa with special reference to Staphylococcus schleiferi subsp. coagulans isolates.
Isolation and characterization of staphylococci from external auditory meatus of dogs with or without otitis externa with special reference to Staphylococcus schleiferi subsp. coagulans isolates.
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从患有或不患有外耳炎的狗的外耳道中分离和鉴定葡萄球菌，特别是施莱菲葡萄球菌亚种。
DOI：
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发表时间：
2005
影响因子：
1.2
通讯作者：
S. Igimi
中科院分区：
文献类型：
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作者：
K. Yamashita;A. Shimizu;J. Kawano;E. Uchida;A. Haruna;S. Igimi
在29只患外耳道炎的犬（OE犬）中有14只（48.3%）和41只未患OE的犬（非OE犬）中有28只（68.3%）从外耳道分离出葡萄球菌。22个OE分离物被鉴定为属于12个种，42个非OE分离物被鉴定为属于13个种。在OE和非OE分离株中发现的优势种是S。intermedius和S.表皮。64株分离株中有38株（59.4%）对17种抗菌药物中的一种或多种耐药。对PCG和ABPC的耐药率最高。S.斯氏亚种从OE和非OE犬中分离出凝结物，其是犬OE的最新病原体。所有的5S。斯氏亚种凝结物分离株具有典型特征。OE和非OE分离株的胞外酶或毒素谱以及PFGE图谱没有明显差异。斯氏亚种凝结物设计了一对16SrDNA特异性PCR引物，用于分离沙门氏菌。斯氏亚种凝结物在所有5个分离株以及模式株GA 211（=JCM 7470）和参考株GA 11中均检测到扩增片段，但在相关种S.金黄色葡萄球菌、intermedius和S.猪。该方法可用于沙门氏菌的简单、快速、准确的鉴定。斯氏亚种凝结物，除了游离凝固酶的标准试管试验。
Staphylococci were isolated from the external auditory meatus in 14 (48.3%) of 29 dogs affected with otitis externa (OE dogs) and 28 (68.3%) of 41 dogs without OE (non-OE dogs). Twenty-two OE isolates were identified as belonging to 12 species, and 42 non-OE isolates were identified as belonging to 13 species. The predominant species found in both OE and non-OE isolates were S. intermedius, and S. epidermidis. Thirty-eight (59.4%) of 64 isolates were resistant to one or more of the 17 antimicrobial agents tested. Resistance to PCG and ABPC was most frequent. S. schleiferi subsp. coagulans, a recent etiologic agent of canine OE, was isolated from OE and non-OE dogs. All of the 5 S. schleiferi subsp. coagulans isolates showed typical characteristics. No clear difference in the extracellular enzyme or toxin profiles, nor in the PFGE patterns, was demonstrated between the OE and non-OE isolates of S. schleiferi subsp. coagulans. A new PCR primer set specific for 16S rDNA was designed to identify strains of S. schleiferi subsp. coagulans. The amplified fragment was detected in all of the 5 isolates as well as the type strain GA 211 (=JCM 7470) and a reference strain GA 11, but was not detected in any strains of the related species, S. aureus, S. intermedius and S. hyicus. The PCR may allow a simple, rapid and precise identification of S. schleiferi subsp. coagulans, in addition to the standard tube test for free coagulase.