Image-based single-cell sorting via dual-photopolymerized microwell arrays.
Image-based single-cell sorting via dual-photopolymerized microwell arrays.
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DOI：
10.1021/ac403777g
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发表时间：
2014-01
影响因子：
7.4
通讯作者：
T. Sun;J. Kovac;J. Voldman
中科院分区：
文献类型：
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作者：
T. Sun;J. Kovac;J. Voldman
我们提出了一个简单的和具有成本效益的单细胞分选方法，使用两个顺序的光聚合步骤，使分选基于成像表型。光聚合的第一步使用基于噻吩的树脂，具有最小的自身荧光，以创建微孔阵列来捕获细胞。第二种光聚合使用（聚）乙二醇二丙烯酸酯将不需要的细胞包封在水凝胶中，允许使用简单的洗涤回收所需的细胞群。我们使用荧光标记细胞定量表征该方法，然后将该方法应用于基于成像荧光定位的细胞分离。该方法易于转移到其他实验室，并将提供一种基于成像表型的细胞分选的简便途径。
We present a simple and cost-effective single-cell sorting method using two sequential photopolymerization steps that enables sorting based upon imaged phenotypes. The first photopolymerization step uses a thiolene-based resin with minimal autofluorescence to create an array of microwells to capture cells. The second photopolymerization uses (poly)ethylene glycol diacrylate to encapsulate undesired cells in a hydrogel, allowing for retrieval of the desired cell population using simple washing. We quantitatively characterize the method using fluorescently labeled cells and then applied the method to isolate cells based upon imaged fluorescence localization. The method is readily transferrable to other laboratories and will provide a facile route to sorting of cells based on imaged phenotypes.