NEDD8-conjugated Cullin4 positive regulates antimicrobial peptides expression in Eriocheir sinensis
NEDD8-conjugated Cullin4 positive regulates antimicrobial peptides expression in Eriocheir sinensis
复制标题
NEDD8 结合的 Cullin4 正调控中华绒螯蟹抗菌肽的表达
DOI：
10.1016/j.fsi.2018.10.077
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发表时间：
2019
影响因子：
4.7
通讯作者：
Li Weiwei
中科院分区：
文献类型：
--
作者：
Yang Lei;Ruan Zechao;Li Xuejie;Li Lu;Wang Qun;Li Weiwei
The ubiquitin-proteasome system is involved in numerous cellular processes, such as signal transduction, autophagy, cell cycle control, embryogenesis, and regulation of immune response. Neural precursor cell expressed developmentally downregulated 8 (NEDD8) is a ubiquitin-like protein that activates Cullin-RING ligases and modifies substrates via neddylation. However, there is limited information on how neddylation regulates innate immunity in crustaceans. In the present study, we identified the evolutionarily conserved NEDD8 with the ubiquitin homologue domain in the Chinese mitten crab (Eriocheir sinensis), named itEsNEDD8. Then, we analyzed the expression patterns and cellular location of its substrate,EsCullin4. qRT-PCR showed that bothEsNEDD8 andEsCullin4 were widely expressed in all the selected tissues, andEsCullin4 was significantly upregulated in hemocytes after bacterial stimulation. Moreover, silencing ofEsCullin4 significantly suppressed the expression of antimicrobial peptides (AMPs) in the hemocytes after bacterial stimulation, and inhibition ofEsCullin4 neddylation by treatment with the NEDD8-activating enzyme inhibitor MLN4924 significantly inhibited the expression of the AMPs. Thus, the results show thatEsNEDD8-modifiedEsCullin4 could control antimicrobial activities via regulation of AMPs expression in the Chinese mitten crab.
