Association between nonsynonymous mutations of starch synthase IIa and starch quality in rice (Oryza sativa)
Association between nonsynonymous mutations of starch synthase IIa and starch quality in rice (Oryza sativa)
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DOI：
10.1111/j.1469-8137.2010.03495.x
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发表时间：
2011-01-01
期刊：
影响因子：
9.4
通讯作者：
Schaal, Barbara A.
中科院分区：
文献类型：
--
作者：
Yu, Guoqin;Olsen, Kenneth M.;Schaal, Barbara A.
淀粉品质是亚洲水稻最重要的农艺性状之一.淀粉合成酶Ⅱ a（Starch synthase IIa，SsIIa）是影响淀粉品质变异的主要候选基因。在SsIIa中，外显子8的三个非同义突变已被证明影响酶活性时，在Escherichiacoli.To搜索的变化，是负责在水稻淀粉品质的变化，我们测序的SsIIa外显子8区域，并测量淀粉品质作为淀粉崩解碱289个品种的栽培稻和57个品种的野生祖先，普通野生稻。采用线性模型和巢式进化枝分析方法对3个非同义单核苷酸多态性（SNPs）与淀粉品质之间的关系进行了研究，发现3个非同义SNPs中有一个位点（SNP 3）与淀粉品质存在关联，该位点对应于781位密码子的Leu/Phe替换。第二个SNP，对应于一个瓦尔/Met替换密码子737，可能会显示出与增加的样本大小的关联。SsIIa酶活性的变化与稻米的凝聚力煮熟时，我们的研究结果是一致的选择更具凝聚力的谷物在驯化的热带粳稻。
P>Starch quality is one of the most important agronomic traits in Asian rice, Oryza sativa. Starch synthase IIa (SsIIa) is a major candidate gene for starch quality variation. Within SsIIa, three nonsynonymous mutations in exon 8 have been shown to affect enzyme activity when expressed in Escherichia coli.To search for the variation in SsIIa that is responsible for starch quality variation in rice, we sequenced the SsIIa exon 8 region and measured starch quality as starch disintegration in alkali for 289 accessions of cultivated rice and 57 accessions of its wild ancestor, Oryza rufipogon. A general linear model and nested clade analysis were used to identify the associations between the three nonsynonymous single nucleotide polymorphisms (SNPs) and starch quality.Among the three nonsynonymous SNPs, we found strong evidence of association at one nucleotide site ('SNP 3'), corresponding to a Leu/Phe replacement at codon 781. A second SNP, corresponding to a Val/Met replacement at codon 737, could potentially show an association with increased sample sizes.Variation in SsIIa enzyme activity is associated with the cohesiveness of rice grains when cooked, and our findings are consistent with selection for more cohesive grains during the domestication of tropical japonica rice.