SPECIFIC AND COOPERATIVE BINDING OF ESCHERICHIA-COLI SINGLE-STRANDED-DNA BINDING-PROTEIN TO MESSENGER-RNA
SPECIFIC AND COOPERATIVE BINDING OF ESCHERICHIA-COLI SINGLE-STRANDED-DNA BINDING-PROTEIN TO MESSENGER-RNA
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DOI：
10.1093/nar/15.13.5241
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发表时间：
1987-07-10
影响因子：
14.9
通讯作者：
HORIE, K
中科院分区：
文献类型：
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作者：
SHIMAMOTO, N;IKUSHIMA, N;HORIE, K
荧光滴定法测定E.大肠杆菌单链DNA结合蛋白与各种RNA的结合表明，该蛋白特异性和协同性地结合其自身的mRNA。这种结合抑制了ssbandblabutnotnusA的体外表达。这种抑制发生在生理浓度的SSB。并对该蛋白在基因调控中的作用进行了讨论。
Fluorometric titration ofE. colisingle-stranded DNA binding protein with various RNAs showed that the protein specifically and cooperatively binds to its own mRNA. The binding inhibitedin vitroexpression ofssbandblabut notnusA. This inhibition takes place at a physiological concentration of SSB. The function of the protein in gene regulation is discussed.