Identification of a third type of lambda light chain in mouse immunoglobulins.
Identification of a third type of lambda light chain in mouse immunoglobulins.
复制标题
小鼠免疫球蛋白中第三种 lambda 轻链的鉴定。
DOI：
10.1073/pnas.78.1.569
复制
发表时间：
1981
影响因子：
11.1
通讯作者：
Eisen,HN
中科院分区：
文献类型：
--
作者：
Azuma,T;Steiner,LA;Eisen,HN
在检查来自四种骨髓瘤蛋白和两种单克隆抗体的轻链的过程中鉴定了第三种类型的小鼠λ链。基于其抗原特性和特征性COOH末端胰蛋白酶肽，这些免疫球蛋白的轻链先前已被暂时鉴定为λ 2链。然而，这些链中的四个，现在被命名为λ 3（或λ III），被发现共享恒定区特征，这清楚地将它们与其他类型的小鼠λ链（λ 1和λ 2）区分开来。测定了λ 3恒定区（从位置120到COOH-末端）90%以上的氨基酸序列。在该区域中，λ 3序列与λ 2恒定区在5个位置不同，与λ 1恒定区在约30个位置不同。
A third type of mouse lambda chain was identified in the course of examining light chains from four myeloma proteins and two monoclonal antibodies. On the basis of their antigenic properties and a characteristic COOH-terminal tryptic peptide, the light chains from these immunoglobulins had previously been provisionally identified as lambda 2 chains. However, four of these chains, designated now as lambda 3 (or lambda III), were found to share constant region features that clearly distinguish them from the other types of mouse lambda chains (lambda 1 and lambda 2). The amino acid sequence of more than 90% of the lambda 3 constant region (from position 120 to the COOH-terminus) was determined. In this region the lambda 3 sequence differs from the lambda 2 constant region at 5 positions and from the lambda 1 constant region at about 30 positions.