Recombinant human stem cell factor enhances the formation of colonies by CD34+ and CD34+lin- cells, and the generation of colony-forming cell progeny from CD34+lin- cells cultured with interleukin-3, granulocyte colony-stimulating factor, or granulocyte-macrophage colony-stimulating factor.
Recombinant human stem cell factor enhances the formation of colonies by CD34+ and CD34+lin- cells, and the generation of colony-forming cell progeny from CD34+lin- cells cultured with interleukin-3, granulocyte colony-stimulating factor, or granulocyte-macrophage colony-stimulating factor.
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重组人干细胞因子可增强 CD34 和 CD34 lin- 细胞集落的形成，以及与白介素-3、粒细胞集落刺激因子或粒细胞-巨噬细胞集落培养的 CD34 lin- 细胞产生集落形成细胞后代。
作者：
I. Bernstein;R. Andrews;K. Zsebo
我们测试了重组人干细胞因子（SCF）刺激分离的骨髓前体细胞在半固体培养基中形成集落和在液体培养中产生集落形成细胞（CFC）的能力。SCF与白细胞介素-3（IL-3）、粒细胞-巨噬细胞集落刺激因子（GM-CSF）或粒细胞集落刺激因子（G-CSF）联合作用，使CD 34+细胞形成数量增加的粒细胞-巨噬细胞集落（CFU-GM），并在IL-3、红细胞生成素（Epo）和SCF的存在下形成宏观红细胞爆发形成单位（BFU-E）。我们测试了分离的CD 34 +lin-细胞，一种不显示与淋巴或骨髓谱系相关的抗原的CD 34+细胞的小亚群，以及包含绝大多数CFC的CD 34 +lin+细胞，并发现增强的集落生长在CD 34 +lin-群体中最显著。在含有SCF与IL-3、GM-CSF或G-CSF组合的液体培养基中培养的CD_（34）+lin-细胞引起CFC数量增加。在培养7 - 21天后，从CD 34 +lin-细胞产生最大数量的CFU-GM，并且从液体培养开始需要SCF的存在。在单个CD 34 +lin-细胞的培养物中，将SCF添加到IL-3和/或G-CSF中导致CFC数量增加，这是由于原本静止的前体细胞的增殖和单个前体细胞产生的CFC数量增加。这些研究证明了SCF和其他造血生长因子对高度不成熟的CD 34 +lin-前体细胞群体的有效协同相互作用。
We tested the ability of recombinant human stem cell factor (SCF) to stimulate isolated marrow precursor cells to form colonies in semisolid media and to generate colony-forming cells (CFC) in liquid culture. SCF, in combination with interleukin-3 (IL-3), granulocyte-macrophage colony-stimulating factor (GM-CSF), or granulocyte colony-stimulating factor (G-CSF) caused CD34+ cells to form increased numbers of granulocyte-macrophage colonies (CFU-GM), and to form macroscopic erythroid burst-forming units (BFU-E) in the presence of IL-3, erythropoietin (Epo), and SCF. We tested isolated CD34+lin- cells, a minor subset of CD34+ cells that did not display antigens associated with lymphoid or myeloid lineages, and CD34+lin+ cells, which contain the vast majority of CFC, and found that the enhanced colony growth was most dramatic within the CD34+lin- population. CD34+lin- cells cultured in liquid medium containing SCF combined with IL-3, GM-CSF, or G-CSF gave rise to increased numbers of CFC. Maximal numbers of CFU-GM were generated from CD34+lin- cells after 7 to 21 days of culture, and required the presence of SCF from the initiation of liquid culture. The addition of SCF to IL-3 and/or G-CSF in cultures of single CD34+lin- cells resulted in increased numbers of CFC due to the proliferation of otherwise quiescent precursors and an increase in the numbers of CFC generated from individual precursors. These studies demonstrate the potent synergistic interaction between SCF and other hematopoietic growth factors on a highly immature population of CD34+lin- precursor cells.
对白细胞介素 3 和粒细胞集落刺激因子的增殖反应区分了一小部分不表达 CD33 和新抗原 7B9 的 CD34 阳性骨髓祖细胞亚群。
影响因子：
20.3
作者：
Litzow,MR;Brashem-Stein,C;Andrews,RG;Bernstein,ID
通讯作者：
Bernstein,ID
抗体同种型对淋巴瘤被动血清疗法的影响。
DOI：
--
发表时间：
1985
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Denkers,EY;Badger,CC;Ledbetter,JA;Bernstein,ID
通讯作者：
Bernstein,ID
能够在体外形成含有母细胞集落的人类造血祖细胞的表征。
DOI：
10.1172/jci113658
发表时间：
1988
期刊：
The Journal of clinical investigation
影响因子：
--
作者：
Brandt,J;Baird,N;Lu,L;Srour,E;Hoffman,R
通讯作者：
Hoffman,R