The earliest intrathymic precursors of CD8α+ thymic dendritic cells correspond to myeloid-type double-negative 1c cells
The earliest intrathymic precursors of CD8α+ thymic dendritic cells correspond to myeloid-type double-negative 1c cells
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DOI：
10.1002/eji.201141728
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发表时间：
2011-08-01
影响因子：
5.4
通讯作者：
Malissen, Bernard
中科院分区：
文献类型：
--
作者：
Luche, Herve;Ardouin, Laurence;Malissen, Bernard
存在于淋巴和非淋巴器官中的树突状细胞（DC）由具有骨髓限制性潜能的祖细胞产生。然而，在胸腺中，表达CD 8 α和langerin（CD 207）的DC的主要亚群似乎与所有其他DC不同，因为它被认为源自具有淋巴潜能的祖细胞。使用表达荧光报告基因和在cd 207基因控制下的白喉毒素受体的小鼠，我们证明了CD 207（+）CD 8 α（+）胸腺DC与T细胞不具有共同的起源，而是来源于胸腺内前体，其表达通常存在于所有（CD 11 c（+）和MHCII分子）或一些（CD 11 c（+）和MHCII分子）上的标记物。（CD 207、CD 135、CD 8 α、CX 3CR 1）DC亚群。这些胸腺内髓样型前体细胞对应于CD 44（+）CD 25（-）双阴性1c（DN 1c）细胞，并从骨髓来源的典型DC前体细胞持续更新。总之，我们的研究结果表明，最早的胸腺内CD 8 α（+）胸腺DC的前体细胞对应于髓样型DN 1c细胞，并支持这样的观点，即在生理条件下，髓样限制性祖细胞产生整个星座的DC存在于身体，包括胸腺。
The dendritic cells (DCs) present in lymphoid and non-lymphoid organs are generated from progenitors with myeloid-restricted potential. However, in the thymus a major subset of DCs expressing CD8 alpha and langerin (CD207) appears to stand apart from all other DCs in that it is thought to derive from progenitors with lymphoid potential. Using mice expressing a fluorescent reporter and a diphtheria toxin receptor under the control of the cd207 gene, we demonstrated that CD207(+)CD8 alpha(+) thymic DCs do not share a common origin with T cells but originate from intrathymic precursors that express markers that are normally present on all (CD11c(+) and MHCII molecules) or on some (CD207, CD135, CD8 alpha, CX3CR1) DC subsets. Those intrathymic myeloid-type precursors correspond to CD44(+)CD25(-) double-negative 1c (DN1c) cells and are continuously renewed from bone marrow-derived canonical DC precursors. In conclusion, our results demonstrate that the earliest intrathymic precursors of CD8 alpha(+) thymic DCs correspond to myeloid-type DN1c cells and support the view that under physiological conditions myeloid-restricted progenitors generate the whole constellation of DCs present in the body including the thymus.