Comparison of g23 gene sequence diversity between Novosphingobium and Sphingomonas phages and phage communities in the floodwater of a Japanese paddy field
Comparison of g23 gene sequence diversity between Novosphingobium and Sphingomonas phages and phage communities in the floodwater of a Japanese paddy field
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DOI：
10.1016/j.soilbio.2008.06.008
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发表时间：
2009-02
影响因子：
9.7
通讯作者：
N. Nakayama;S. Asakawa;M. Kimura
中科院分区：
文献类型：
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作者：
N. Nakayama;S. Asakawa;M. Kimura
我们以前的研究表明，从日本稻田的洪水中分离的新鞘氨醇杆菌和鞘氨醇单胞菌属菌株的T4型噬菌体的主要衣壳基因（g23）的多样性与从其他日本稻田获得的克隆相当。为了更严格的多样性比较，本研究探讨了g23序列之间的新鞘氨醇菌和鞘氨醇单胞菌和噬菌体社区在同一洪水的日本稻田。通过对从洪水中提取的DNA应用g23特异性引物获得克隆。洪水中的许多23个克隆被分组为具有新鞘氨醇杆菌/鞘氨醇单胞菌g23基因的水稻组I-VI的相同簇，其中一些克隆属于另外的簇。此外，其余克隆属于海洋克隆和T4型肠噬菌体群。这些结果表明，在洪水中的g23基因比那些新鞘氨醇菌/鞘氨醇单胞菌的g23基因更多样化，包括g23基因密切相关的肠道和海洋起源的基因。
Our previous study indicated that the diversity of the major capsid gene (g23) of T4-type bacteriophages (phages) of Novosphingobium and Sphingomonas strains isolated from the floodwater of a Japanese paddy field is comparable to those of the clones obtained from other Japanese paddy fields. For more strict comparison of the diversity, this study examined g23 sequences between Novosphingobium and Sphingomonas phages and phage communities in the identical floodwater of a Japanese paddy field. The clones were obtained by applying g23-specific primers to DNA extracted from the floodwaters. Many 23 clones in the floodwater were grouped into the same clusters of Paddy Groups I–VI with g23 genes of Novosphingobium/Sphingomonas phages with some clones belonging to an additional cluster. In addition, the remaining clones belonged to the clusters of marine clones and T4-type enterophages. These findings indicate that the g23 genes in the floodwater are more diversified than those of Novosphingobium/Sphingomonas phages including g23 genes closely related to the genes of enterophages and marine origins.