Articular chondrocyte apoptosis in equine osteoarthritis
Articular chondrocyte apoptosis in equine osteoarthritis
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DOI：
10.1016/s1090-0233(02)00305-2
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发表时间：
2003-07-01
影响因子：
2.2
通讯作者：
Cho, DY
中科院分区：
文献类型：
--
作者：
Kim, DY;Taylor, HW;Cho, DY
骨关节炎（OA）是马最常见的关节疾病。软骨细胞凋亡被认为是人类和实验动物 OA 的主要病理变化，但尚未对马 OA 进行研究。从三匹正常马和五匹 OA 马身上采集关节软骨。通过改良的Mankin分级系统对组织病理学变化进行评分。进行末端脱氧核苷酸转移酶介导的 dUTP 缺口末端标记 (TUNEL) 测定来鉴定软骨细胞凋亡。使用硝基酪氨酸多克隆抗体通过免疫组织化学间接评估软骨细胞产生的一氧化氮 (NO)。 OA软骨的组织病理学评分和软骨细胞凋亡百分比显着高于正常软骨。组织病理学分级与软骨细胞凋亡百分比之间存在显着相关性。 OA软骨对硝基酪氨酸的免疫反应性比正常软骨更强。马 OA 软骨中软骨细胞凋亡、软骨基质变性和 NO 产生的拓扑分布重叠，表明这些病理现象密切相关。 (C) 2003 Elsevier Science Ltd. 保留所有权利。
Osteoarthritis (OA) is the most common joint disease in horses. Chondrocyte apoptosis has been implicated as a major pathological OA change in humans and experimental animals but no studies have been performed on equine OA. Articular cartilage was collected from three normal and five OA horses. Histopathological changes were scored by a modified Mankin grading system. A terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL) assay was performed to identify chondrocyte apoptosis. Nitric oxide (NO) production from chondrocytes was indirectly evaluated by immunohistochemistry with polyclonal antibody to nitrotyrosine. The histopathological score and percentage of chondrocyte apoptosis from the OA cartilages were significantly higher than from normal cartilages. There was a significant correlation between histopathological grade and the percentage of chondrocyte apoptosis. OA cartilages exhibited stronger immunoreactivity to nitrotyrosine than normal cartilage. Topographical distributions of chondrocyte apoptosis, cartilage matrix degeneration, and NO production overlapped in equine OA cartilages, suggesting that these pathological phenomena are closely interrelated. (C) 2003 Elsevier Science Ltd. All rights reserved.