CHARACTERIZATION OF MYOCARDIAL PROTEIN-COMPOSITION IN DILATED CARDIOMYOPATHY BY 2-DIMENSIONAL GEL-ELECTROPHORESIS
CHARACTERIZATION OF MYOCARDIAL PROTEIN-COMPOSITION IN DILATED CARDIOMYOPATHY BY 2-DIMENSIONAL GEL-ELECTROPHORESIS
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DOI：
10.1093/eurheartj/15.suppl_d.37
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发表时间：
1994-12-01
影响因子：
39.3
通讯作者：
FLECK, E
中科院分区：
文献类型：
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作者：
KNECHT, M;REGITZZAGROSEK, V;FLECK, E
为了确定扩张型心肌病（DCM）的心肌蛋白质模式的变化特征，我们比较了，通过二维凝胶电泳，右心房蛋白质模式，从5例DCM和4个正常的左心室功能（两个凝胶每个病人）。使用计算机辅助分析（PDQUEST; 4.1），我们在18个凝胶（23 × 30 cm，pH 4-9，分子量10-150 kDa）中发现了可再现的蛋白质模式。在来自同一患者的两个凝胶中，91%的蛋白质在其图案中的位置相同，并且这些蛋白质种类的相对强度与r = 0.85相关。在几种凝胶中发现了302种+/-50蛋白质种类，在所有18种凝胶中发现了186种。DCM组中7种蛋白的相对强度降低> 100%，6种蛋白的相对强度增加> 100%。DCM和对照患者之间的显着定量差异被发现为25种蛋白质。基于七种外部标记蛋白，可以计算每种蛋白的pH和分子量值。到目前为止，已经通过抗体、氨基酸分析或测序程序鉴定了30种蛋白质。从25种蛋白质中，DCM和对照组之间存在显著差异，已鉴定出三种：线粒体肌酸激酶和α-嵴蛋白B链的表达在DCM中显著增加;苹果酸脱氢酶家族在DCM中也显著减少。双向电泳似乎是一个强大的方法，用于检测疾病相关的心肌蛋白质模式的改变。
In order to identify alterations in the myocardial protein pattern that characterize dilated cardiomyopathy (DCM), we compared, by two-dimensional gel electrophoresis, right atrial protein patterns from five patients with DCM and four with normal left ventricular function (two gels per patient). Using computer assisted analysis (PDQUEST; 4.1) we found reproducible protein patterns in the 18 gels (23 x 30 cm, pH 4-9, molecular weight 10-150 kDa). In the two gels from the same patient, 91 % of proteins were identical in their position in the pattern and the relative intensities of these protein species correlated with r = 0.85. Three hundred and two +/- 50 protein species were found in several gels, 186 in all 18 gels. Seven proteins in the DCM group were decreased in their relative intensity by > 100%, six were increased by >100%. Significant quantitative differences between DCM and control patients were found for 25 protein species. Based on seven external marker proteins, a pH and molecular weight value could be calculated for each protein. So far, 30 protein species have been identified by antibodies, amino acid analysis or sequencing procedures. From the 25 proteins that are significantly different between DCM and controls, three have been identified Expression of the mitochondrial creatine kinase and alpha cristallin B chain was significantly increased in DCM; the malate dehydrogenase family was also significantly decreased in DCM. Two-dimensional electrophoresis appears to be a powerful method for the detection of disease-associated alterations in the myocardial protein pattern.