Biological response and morphological assessment of individually dispersed multi-wall carbon nanotubes in the lung after intratracheal instillation in rats
Biological response and morphological assessment of individually dispersed multi-wall carbon nanotubes in the lung after intratracheal instillation in rats
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DOI：
10.1016/j.tox.2010.07.021
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发表时间：
2010-10-29
期刊：
影响因子：
4.5
通讯作者：
Nakanishi, Junko
中科院分区：
文献类型：
--
作者：
Kobayashi, Norihiro;Naya, Masato;Nakanishi, Junko
多壁碳纳米管（MWCNTs）的生物学反应进行了评估后，一个单一的大鼠肠道滴注。在这项研究中使用的多壁碳纳米管的直径和中值长度分别约为60 nm和1.5 μ m。向各组雄性Sprague-Dawley大鼠的气管内滴注0.04、0.2或1 mg/kg单独分散的MWCNT混悬液。滴注后，评估支气管肺泡灌洗液中的炎性细胞和标志物，并在暴露后3天、1周、1个月、3个月和6个月对肺、肝、肾、脾和大脑进行组织病理学评价。仅在暴露于1 mg/kg MWCNT的大鼠组的肺中观察到短暂的肺部炎症反应。使用光学显微镜和透射电子显微镜（TEM）评估大鼠肺中灌注的多壁碳纳米管的形态。光镜检查显示，沉积在大鼠肺中的多壁碳纳米管通常被肺泡巨噬细胞吞噬，这些巨噬细胞因此积累在肺泡中，直到暴露后6个月。400张透射电镜图像显示，所有多壁碳纳米管均位于肺泡巨噬细胞或间质组织中的巨噬细胞中，而多壁碳纳米管未位于间质组织的细胞中。没有证据表明多壁碳纳米管滴注会诱导慢性炎症，例如血管生成或纤维化。这些结果表明，多壁碳纳米管被肺泡巨噬细胞加工和清除。(C)2010爱思唯尔爱尔兰有限公司版权所有。
Biological responses of multi-wall carbon nanotubes (MWCNTs) were assessed after a single intratracheal instillation in rats. The diameter and median length of the MWCNTs used in this study were approximately 60 nm and 1.5 mu m, respectively. Groups of male Sprague-Dawley rats were intratracheally instilled with 0.04, 0.2, or 1 mg/kg of the individually dispersed MWCNT suspension. After instillation, the bronchoalveolar lavage fluid was assessed for inflammatory cells and markers, and the lung, liver, kidney, spleen, and cerebrum were histopathologically evaluated at 3-day, 1-week, 1-month, 3-month, and 6-month post-exposure. Transient pulmonary inflammatory responses were observed only in the lungs of the group of rats exposed to 1 mg/kg of MWCNTs. Morphology of the instilled MWCNTs in the lungs of rats was assessed using light microscopy and transmission electron microscopy (TEM). Light microscopy examination revealed that MWCNTs deposited in the lungs of the rats were typically phagocytosed by the alveolar macrophages and these macrophages were consequently accumulated in the alveoli until 6-month post-exposure. The 400 TEM images obtained showed that all MWCNTs were located in the alveolar macrophages or macrophages in the interstitial tissues, and MWCNTs were not located in the cells of the interstitial tissues. There was no evidence of chronic inflammation, such as angiogenesis or fibrosis, induced by MWCNT instillation. These results suggest that MWCNTs were being processed and cleared by alveolar macrophages. (C) 2010 Elsevier Ireland Ltd. All rights reserved.