A rapid method for chromatographic analysis of oligosaccharides on Bio-Gel P-4.
A rapid method for chromatographic analysis of oligosaccharides on Bio-Gel P-4.
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在 Bio-Gel P-4 上对寡糖进行快速色谱分析的方法。
DOI：
10.1016/0003-2697(80)90439-x
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发表时间：
1980
影响因子：
2.9
通讯作者：
Baenziger,JU
中科院分区：
文献类型：
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作者：
Natowicz,M;Baenziger,JU
我们开发了一种在Bio-Gel P-4（-400目）上通过色谱分离快速分析低聚糖及其降解产物的方法。在55-100 psi下，使用0.6 × 100 cm的单柱或两个0.6 × 100 cm的耦合柱，可以达到64 ml cm−2/h− 1的流速，分别允许在1.5或3.0 h内进行分析。对于至少大至15个葡萄糖当量的低聚物，可以精确区分1个葡萄糖当量的差异。证明了该方法用于糖蛋白衍生寡糖分析的适用性。通过在这样的寡糖中赋予每个中性糖残基1葡萄糖当量和每个氨基糖残基2葡萄糖当量的值，可以快速和精确地监测各种外切糖苷酶和内切糖苷酶二聚体的作用。这些色谱柱非常稳定，可连续运行至少9个月，用于300多个样品而不发生劣化。
We have developed a method for the rapid analysis of oligosaccharides and their degradation products by chromatographic separation on Bio-Gel P-4 (−400 mesh). Flow rates of 64 ml cm−2/h−1can be attained at 55–100 psi with either a single column of 0.6 × 100 cm or two coupled columns each 0.6 × 100 cm, permitting analyses to be carried out in 1.5 or 3.0 h, respectively. Differences of 1 glucose equivalent can be precisely distinguished for oligomers at least as large as 15 glucose equivalents. The applicability of this method for the analysis of glycoprotein-derived oligosaccharides is demonstrated. By assigning a value of 1 glucose equivalent per neutral sugar residue and 2 glucose equivalents per amino sugar residue in such oligosaccharides, the effect of various exo- and endoglycosidase digestions can be rapidly and precisely monitored. These columns are extremely stable, providing continuous operation for at least 9 months with over 300 sample applications without deterioration.