Behaviour-dependent recruitment of long-range projection neurons in somatosensory cortex
Behaviour-dependent recruitment of long-range projection neurons in somatosensory cortex
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DOI：
10.1038/nature12236
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发表时间：
2013-07-18
期刊：
影响因子：
64.8
通讯作者：
Helmchen, Fritjof
中科院分区：
文献类型：
--
作者：
Chen, Jerry L.;Carta, Stefano;Helmchen, Fritjof
在哺乳动物的新大脑皮层中，分离的加工流被认为对形成对环境的感觉表征(1，2)很重要，但在行为过程中，初级感觉皮质中的局部信息如何传递到其他遥远的皮质区域尚不清楚。在这里，我们展示了清醒的、行为正常的小鼠初级躯体感觉皮质(S1)胡须区域中不同的、基本上不重叠的远程投射神经元的任务依赖激活。使用双光子钙成像，我们监测了解剖学上识别的投射到小鼠次级躯体感觉(S2)或初级运动(M1)皮质的S1神经元的神经元活动，使用它们的胡须执行纹理识别任务或要求它们检测特定位置是否存在物体的任务。在S2投射(S2P)神经元中发现了与甩动相关的细胞，但在M1投射(M1P)神经元中未发现与扭动相关的细胞。在纹理识别中，S2P神经元比M1P神经元表现出更高比例的触摸相关反应，而在检测任务中，M1P神经元比S2P神经元表现出更高比例的触摸相关反应。在这两项任务中，S2P和M1P神经元可以在产生不同行为决定的试验中进行类似的区分。然而，在产生相同决定的试验中，S2P神经元在区分纹理方面表现得更好，而M1P神经元在区分位置方面表现更好。仅靠感觉刺激特征不足以引起这些差异，这表明S1信息向S2和M1的选择性传输是由行为驱动的。
In the mammalian neocortex, segregated processing streams are thought to be important for forming sensory representations of the environment(1,2), but how local information in primary sensory cortex is transmitted to other distant cortical areas during behaviour is unclear. Here we show task-dependent activation of distinct, largely non-overlapping long-range projection neurons in the whisker region of primary somatosensory cortex (S1) in awake, behaving mice. Using two-photon calcium imaging, we monitored neuronal activity in anatomically identified S1 neurons projecting to secondary somatosensory (S2) or primary motor (M1) cortex in mice using their whiskers to perform a texture-discrimination task or a task that required them to detect the presence of an object at a certain location. Whisking-related cells were found among S2-projecting (S2P) but not M1-projecting (M1P) neurons. A higher fraction of S2P than M1P neurons showed touch-related responses during texture discrimination, whereas a higher fraction of M1P than S2P neurons showed touch-related responses during the detection task. In both tasks, S2P and M1P neurons could discriminate similarly between trials producing different behavioural decisions. However, in trials producing the same decision, S2P neurons performed better at discriminating texture, whereas M1P neurons were better at discriminating location. Sensory stimulus features alone were not sufficient to elicit these differences, suggesting that selective transmission of S1 information to S2 and M1 is driven by behaviour.