Optimization of recombinant protein expression in the chloroplasts of green algae.
Optimization of recombinant protein expression in the chloroplasts of green algae.
复制标题
绿藻叶绿体中重组蛋白表达的优化。
DOI：
10.1007/978-0-387-75532-8_8
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发表时间：
2007
影响因子：
--
通讯作者：
Mayfield,StephenP
中科院分区：
文献类型：
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作者：
Fletcher,SamuelP;Muto,Machiko;Mayfield,StephenP
通过分子和遗传技术的进步，绿藻叶绿体中的蛋白质表达已经得到优化，以实现高水平的表达。在藻类中表达的重组蛋白有可能为疾病和感染提供新颖和安全的治疗方法，在目前的高成本药物是唯一选择的情况下，或者更糟糕的是，在治疗药物由于制造成本高得令人望而却步而无法获得的情况下。利用叶绿体密码子偏置和启动子与非编码区组合的组合检验，优化了衣藻重组蛋白在叶绿体中的表达。此外，抗疱疹病毒抗体的表达表明，C。重组叶绿体能够正确折叠和组装复杂的哺乳动物蛋白质。这些数据确立了藻类叶绿体作为一种系统，用于生产可溶性和活性形式的复杂人类治疗性蛋白，并且与现有的生产系统相比，大大减少了时间和成本。在藻类叶绿体中生产重组蛋白可能使进一步开发安全、有效和经济的蛋白质疗法成为可能。
Through advances in molecular and genetic techniques, protein expression in the chloroplasts of green algae has been optimized for high-level expression. Recombinant proteins expressed in algae have the potential to provide novel and safe treatment of disease and infection where current, high-cost drugs are the only option, or worse, where therapeutic drugs are not available due to their prohibitively high-cost to manufacture. Optimization of recombinant protein expression inChlamydomonas reinhardtiichloroplasts has been accomplished by employing chloroplast codon bias and combinatorial examination of promoter and UTR combinations. In addition, as displayed by the expression of an anti-herpes antibody, theC. reinhardtiichloroplast is capable of correctly folding and assembling complex mammalian proteins. These data establish algal chloroplasts as a system for the production of complex human therapeutic proteins in soluble and active form, and at significandy reduced time and cost compared to existing production systems. Production of recombinant proteins in algal chloroplasts may enable further development of safe, efficacious and cost-effective protein therapeutics.