IDentification and Analysis of the Arabidopsis Thaliana Atfas4 Gene Whose Overexpression Results in the Development of A Fasciated Stem.
IDentification and Analysis of the Arabidopsis Thaliana Atfas4 Gene Whose Overexpression Results in the Development of A Fasciated Stem.
复制标题
拟南芥ATFAS4基因的鉴定和分析，其过表达导致着迷的茎的发展。
DOI：
10.4172/jpb.1000041
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发表时间：
2008-10
期刊：
影响因子：
--
通讯作者：
Klimov E
中科院分区：
文献类型：
--
作者：
Pogorelko G;Fursova O;Klimov E
使用一种新的pEnLox载体，用于在拟南芥中产生功能获得性突变体，AtFAS 4突变体已经获得并分析。该突变体的特征在于At 1g 33390基因的超表达，这导致突变表型-茎筋膜化的发生。AtFAS 4基因在正常发育的拟南芥植物中的表达水平极低，因此解释了关于该基因的EST的信息几乎完全缺失。所产生的AtFAS 4突变体允许获得At 1g 33390基因的全长cDNA并首次进行分析。
Using a new pEnLox vector employed to generate gain-of-function mutants in Arabidopsis thaliana, the AtFAS4 mutant has been obtained and analyzed. The mutant is characterized by super-expression of the At1g33390 gene, which leads to the occurrence of a mutant phenotype – stem fasciation. The level of expression of the AtFAS4 gene in normally developing A.thaliana plants is extremely low thus accounting for almost complete absence of information on EST’s of this gene. The generated AtFAS4 mutant has permitted full-length cDNA of the At1g33390 gene to be obtained and analyzed for the first time.