CLONING OF CDNA SEQUENCES OF HORMONE-REGULATED GENES FROM THE MCF-7 HUMAN-BREAST CANCER CELL-LINE
CLONING OF CDNA SEQUENCES OF HORMONE-REGULATED GENES FROM THE MCF-7 HUMAN-BREAST CANCER CELL-LINE
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DOI：
10.1093/nar/10.24.7895
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发表时间：
1982-01-01
影响因子：
14.9
通讯作者：
CHAMBON, P
中科院分区：
文献类型：
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作者：
MASIAKOWSKI, P;BREATHNACH, R;CHAMBON, P
通过对含有雌激素受体的乳腺癌细胞系MCF-7的cDNA文库进行差异筛选，分离出了对应于mRNA的cDNA克隆，所述mRNA的水平通过向培养基中加入雌二醇而迅速增加。这些克隆将可用于研究激素诱导所需的DNA序列，并确定相应基因的表达是否以任何方式与癌状态相关。我们还获得了一个信使的cDNA克隆，该信使的水平明显地被类固醇激素降低
cDNA clones corresponding to a mRNA whose level is rapidly increased by addition of oestradiol to the culture medium have been isolated by differential screening of a cDNA library from the breast cancer cell line MCF-7, which contains oestrogen receptors. Such clones will be useful in studies of the DNA sequences required for hormonal induction and to determine whether expression of the corresponding gene is in any way related to the cancerous state. We have also obtained a cDNA clone for a messenger whose level is apparently decreased by steroid hormones