Vitamin E against oxidative damage caused by formaldehyde in frontal cortex and hippocampus: Biochemical and histological studies
Vitamin E against oxidative damage caused by formaldehyde in frontal cortex and hippocampus: Biochemical and histological studies
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DOI：
10.1016/j.jchemneu.2005.01.001
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发表时间：
2005-05-01
影响因子：
2.8
通讯作者：
Ozen, OA
中科院分区：
文献类型：
--
作者：
Gurel, A;Coskun, O;Ozen, OA
甲醛(FA)可导致严重的中枢神经系统损害。但是，关于FA中毒引起的额叶皮质和海马区组织的生化和组织病理学改变的研究还很少。本研究旨在探讨慢性甲醛中毒后额叶皮质和海马区组织的变化及维生素E(VitE)对氧化损伤的保护作用。18只大鼠分为3组：(1)对照组，(2)FA组(FAT)，(3)FA+VitE组(FAT+VitE)。治疗结束后处死动物，取额叶皮质和海马区组织进行生化和组织病理学观察。与对照组相比，黄腐酸可显著提高组织丙二醛(MDA)和蛋白质羰基(PC)水平，降低额叶皮质和海马区超氧化物歧化酶(SOD)和过氧化氢酶(CAT)的活性。维生素E处理降低了组织中丙二醛和PC的水平，并阻止了对组织中超氧化物歧化酶和过氧化氢酶的抑制。在脂肪组，两个组织的神经元广泛变暗，变性，核团突触。脂肪+维生素E组神经元形态受到较好的保护，但不如对照组。脂肪组额叶皮质和海马区神经元数量明显少于对照组和脂肪+维生素E组。结论：维生素E治疗可能有助于预防FA所致的额叶皮质和海马区组织的氧化损伤，具有临床应用潜力。(C)2005 Elsevier B.V.保留所有权利。
Formaldehyde (FA) can cause severe central nervous system impairment. But, there are only a few studies about biochemical and histopathological changes of frontal cortex and hippocampal tissue caused by FA toxicity. The aim of our study was to investigate these changes occurring after chronic formaldehyde toxicity in frontal cortex and hippocampal tissues, and protective effect of Vitamin E (vit E) against oxidative damage. Eighteen rats were divided into three groups: (1) control, (2) treated with FA (FAt), and (3) treated with FA and vit E (FAt + vit E) groups. After the treatment, the animals were sacrificed and frontal cortex and hippocampal tissues were removed for biochemical and histopathological investigation. FA significantly increased tissue malondialdehyde (MDA) and protein carbonyl (PC) levels and also decreased superoxide dismutase (SOD) and catalase (CAT) enzyme activities in frontal cortex and hippocampal tissue compared to control. Vit E treatment decreased MDA and PC levels and prevented inhibition of SOD and CAT enzymes in the tissues. In the FAt group, the neurons of both tissues became extensively dark and degenerated with picnotic nuclei. The morphology of neurons in FAt + vit E group was protected well, but not as neurons of the control group. The number of neurons in frontal cortex and hippocampal tissue of FAt group was significantly less than both control and FAt + vit E groups. It was concluded that vit E treatment might be beneficial in preventing FA-induced oxidative frontal cortex and hippocampal tissue damage, therefore, shows potential for clinical use. (c) 2005 Elsevier B.V. All rights reserved.