Methicillin (oxacillin)-resistant Staphylococcus aureus strains isolated from major food animals and their potential transmission to humans
Methicillin (oxacillin)-resistant Staphylococcus aureus strains isolated from major food animals and their potential transmission to humans
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DOI：
10.1128/aem.69.11.6489-6494.2003
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发表时间：
2003-11-01
影响因子：
4.4
通讯作者：
Lee, JH
中科院分区：
文献类型：
--
作者：
Lee, JH
从2001年5月至2003年4月，从牛、猪和鸡中采集各种类型的标本，并检查是否存在耐甲氧西林（苯唑西林）金黄色葡萄球菌（MRSA）。S.分离金黄色葡萄球菌，并通过革兰氏染色、菌落形态、凝固酶和尿素酶活性试验以及API StaphIdent系统进行阳性鉴定。在1,913份动物标本中，421份含有S。金黄色葡萄球菌;其中，28个含有S.金黄色葡萄球菌耐苯唑西林浓度高于2 μ g/ml。28份标本中有15份mecA基因PCR阳性。在15株mecA阳性MRSA分离株中，12株来自奶牛，3株来自鸡。采用纸片扩散法对mecA阳性MRSA菌株进行药敏试验。所有分离株对青霉素家族的成员，如氨苄西林、苯唑西林和青霉素均具有耐药性。所有分离株也敏感阿米卡星，万古霉素，和甲氧苄啶-磺胺甲恶唑。为了确定这15种动物MRSA分离株与人类分离株的分子流行病学相关性，通过任意引物PCR产生随机扩增多态性DNA（RAPD）图谱。其中6株动物分离株的RAPD图谱与某些人分离株的图谱完全相同。6株动物分离株的DNA分型与人分离株的DNA分型相似。这些数据表明，六种动物MRSA分离株的基因组与一些人类MRSA分离株的基因组非常密切相关，并且可能是因食用这些动物制成的受污染食品而引起的人类感染的来源。
From May 2001 to April 2003, various types of specimens from cattle, pigs, and chickens were collected and examined for the presence of methicillin (oxacillin) -resistant Staphylococcus aureus (MRSA). S. aureus was isolated and positively identified by using Gram staining, colony morphology, tests for coagulase and urease activities, and an API Staph Ident system. Among 1,913 specimens collected from the animals, 421 contained S. aureus; of these, 28 contained S. aureus resistant to concentrations of oxacillin higher than 2 mug/ml. Isolates from 15 of the 28 specimens were positive by PCR for the mecA gene. Of the 15 mecA-positive MRSA isolates, 12 were from dairy cows and 3 were from chickens. Antimicrobial susceptibility tests of mecA-positive MRSA strains were performed by the disk diffusion method. All isolates were resistant to members of the penicillin family, such as ampicillin, oxacillin, and penicillin. All isolates were also susceptible to amikacin, vancomycin, and trimethoprim-sulfamethoxazole. To determine molecular epidemiological relatedness of these 15 animal MRSA isolates to isolates from humans, random amplified polymorphic DNA (RAPD) patterns were generated by arbitrarily primed PCR. The RAPD patterns of six of the isolates from animals were identical to the patterns of certain isolates from humans. The antibiotypes of the six animal isolates revealed types similar to those of the human isolates. These data suggested that the genomes of the six animal MRSA isolates were very closely related to those of some human MRSA isolates and were a possible source of human infections caused by consuming contaminated food products made from these animals.