Isolation of a cell-surface receptor for chick neural retina adherons
Isolation of a cell-surface receptor for chick neural retina adherons
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鸡神经视网膜粘附细胞表面受体的分离
DOI：
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发表时间：
1985
影响因子：
7.8
通讯作者：
M. Lacorbiere
中科院分区：
文献类型：
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作者：
D. Schubert;M. Lacorbiere
鸡胚神经视网膜细胞释放糖蛋白复合物，称为粘附素，进入其培养基。当被吸收到培养皿表面时，神经视网膜粘附素增加神经视网膜细胞粘附的初始速率。在溶液中，它们增加细胞-细胞聚集的速率。培养的视网膜细胞的细胞-细胞和粘附素-细胞粘附被硫酸乙酰肝素糖胺聚糖选择性地抑制，但不被硫酸软骨素或透明质酸，这表明硫酸乙酰肝素蛋白聚糖可能参与粘附过程。我们从神经视网膜细胞的生长条件培养基中分离出硫酸乙酰肝素蛋白聚糖，并制备了抗血清，这些抗体的单价Fab'片段完全抑制细胞与粘附素的粘附，并部分阻断自发的细胞-细胞聚集。从细胞表面分离出抗原性和结构相似的硫酸乙酰肝素蛋白聚糖。这种蛋白聚糖直接与粘附素结合，当被吸收到塑料上时，刺激细胞与基质的粘附。这些数据表明，鸡神经视网膜细胞表面上的硫酸乙酰肝素蛋白聚糖作为受体的粘附介导的糖蛋白复合物（粘附素）。
Embryonic chick neural retina cells release glycoprotein complexes, termed adherons, into their culture medium. When absorbed onto the surface of petri dishes, neural retina adherons increase the initial rate of neural retina cell adhesion. In solution they increase the rate of cell-cell aggregation. Cell-cell and adheron-cell adhesions of cultured retina cells are selectively inhibited by heparan-sulfate glycosaminoglycan, but not by chondroitin sulfate or hyaluronic acid, suggesting that a heparan-sulfate proteoglycan may be involved in the adhesion process. We isolated a heparan-sulfate proteoglycan from the growth-conditioned medium of neural retina cells, and prepared an antiserum against it. Monovalent Fab' fragments of these antibodies completely inhibited cell-adheron adhesion, and partially blocked spontaneous cell-cell aggregation. An antigenically and structurally similar heparan-sulfate proteoglycan was isolated from the cell surface. This proteoglycan bound directly to adherons, and when absorbed to plastic, stimulated cell-substratum adhesion. These data suggest that a heparan-sulfate proteoglycan on the surface of chick neural retina cells acted as a receptor for adhesion-mediating glycoprotein complexes (adherons).