Multispectral confocal microendoscope for in vivo and in situ imaging.
Multispectral confocal microendoscope for in vivo and in situ imaging.
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DOI：
10.1117/1.2950313
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发表时间：
2008-07
影响因子：
3.5
通讯作者：
Rouse AR
中科院分区：
文献类型：
--
作者：
Makhlouf H;Gmitro AF;Tanbakuchi AA;Udovich JA;Rouse AR
我们描述了多光谱共焦显微内窥镜的设计和操作。这种基于光纤的荧光成像系统由耦合到成像导管的狭缝扫描共聚焦显微镜组成，该成像导管被设计为微创并允许体内细胞水平成像。该系统可以在两种成像模式下操作。灰度操作模式提供高分辨率实时体内图像，显示来自样本的荧光信号强度。多光谱操作模式使用棱镜作为色散元件来收集荧光发射的完整多光谱图像。该仪器几乎可以瞬间在两种成像模式之间来回切换（不到半秒）。在目前的配置中，多光谱共焦显微内窥镜达到3 μm的横向分辨率和30 μm的轴向分辨率。该系统记录500至750 nm的光，在此光谱范围内，最小可分辨波长差为2.9至8.3 nm。从离体人体组织和小动物组织的灰度和多光谱成像结果。
We describe the design and operation of a multispectral confocal microendoscope. This fiber-based fluorescence imaging system consists of a slit-scan confocal microscope coupled to an imaging catheter that is designed to be minimally invasive and allow for cellular level imaging in vivo. The system can operate in two imaging modes. The grayscale mode of operation provides high resolution real-time in vivo images showing the intensity of fluorescent signal from the specimen. The multispectral mode of operation uses a prism as a dispersive element to collect a full multispectral image of the fluorescence emission. The instrument can switch back and forth nearly instantaneously between the two imaging modes (less than half a second). In the current configuration, the multispectral confocal microendoscope achieves 3-μm lateral resolution and 30-μm axial resolution. The system records light from 500 to 750 nm, and the minimum resolvable wavelength difference varies from 2.9 to 8.3 nm over this spectral range. Grayscale and multispectral imaging results from ex-vivo human tissues and small animal tissues are presented.