Flexible material formulations for 3D printing of ordered porous beds with applications in bioprocess engineering
Flexible material formulations for 3D printing of ordered porous beds with applications in bioprocess engineering
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DOI：
10.1186/s40643-022-00511-9
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发表时间：
2022-03-12
影响因子：
4.6
通讯作者：
Christofi, Nick
中科院分区：
文献类型：
--
作者：
Dimartino, Simone;Galindo-Rodriguez, Giuseppe Rafael;Christofi, Nick
背景3D打印正在革新许多工业领域，并有潜力增强生物技术和生物加工领域。在这里，我们提出了一种新的柔性材料配方，用于3D打印具有复杂，完美有序形态和可调特性的支撑基质，以适应生物工艺工程中的一系列应用。结果支持制造功能单体作为关键成分，使矩阵与定制的化学，如带电基团，化学部分进一步官能化，和疏水/亲水基团。其它成分，例如交联剂和致孔剂，可用于制造具有其多孔网络的不同特性的载体，提供进一步调节载体的机械和传质性质的机会。通过这种方法，我们制作和演示了Schoen gyroid柱的操作，（I）正电荷和负电荷的离子交换色谱，（II）酶生物反应器与固定化胰蛋白酶催化水解，和（III）细菌生物膜生物反应器燃料脱硫。这项研究证明了一种简单，具有成本效益和灵活的定制3D打印支持的制造方法，可用于不同的生物技术和生物工程应用。
Background 3D printing is revolutioning many industrial sectors and has the potential to enhance also the biotechnology and bioprocessing fields. Here, we propose a new flexible material formulation to 3D print support matrices with complex, perfectly ordered morphology and with tuneable properties to suit a range of applications in bioprocess engineering. Findings Supports were fabricated using functional monomers as the key ingredients, enabling matrices with bespoke chemistry, such as charged groups, chemical moieties for further functionalization, and hydrophobic/hydrophilic groups. Other ingredients, e.g. crosslinkers and porogens, can be employed to fabricate supports with diverse characteristics of their porous network, providing an opportunity to further regulate the mechanical and mass transfer properties of the supports. Through this approach, we fabricated and demonstrated the operation of Schoen gyroid columns with (I) positive and negative charges for ion exchange chromatography, (II) enzyme bioreactors with immobilized trypsin to catalyse hydrolysis, and (III) bacterial biofilm bioreactors for fuel desulphurization. Conclusions This study demonstrates a simple, cost-effective, and flexible fabrication of customized 3D printed supports for different biotechnology and bioengineering applications.