Coculturing Bacteria Leads to Reduced Phenotypic Heterogeneities
Coculturing Bacteria Leads to Reduced Phenotypic Heterogeneities
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DOI：
10.1128/aem.02814-18
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发表时间：
2019-04-01
影响因子：
4.4
通讯作者：
Boon, Nico
中科院分区：
文献类型：
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作者：
Heyse, Jasmine;Buysschaert, Benjamin;Boon, Nico
已知同基因细菌群体在单细胞水平上表现出表型异质性。由于难以评估一个单一的类群在混合社区的表型异质性，这种更深层次的组织的重要性仍然是相对未知的自然社区。在这项研究中，我们使用了基于膜的微观世界，允许探测的表型异质性的一个单一的分类群，同时与合成或天然社区。在无菌条件下，作为物理分离的共培养物的成员，以及作为混合培养物，对单个分类群进行了研究。通过流式细胞术和拉曼光谱评估表型异质性。使用这种设置，我们研究了微生物相互作用对两个相互作用的饮用水分离株的个体表型异质性的影响。通过流式细胞仪，我们已经证明，这些细菌之间的相互作用，导致减少其个人的表型diform，这种调整是有条件的细菌taxon. Single-cell拉曼光谱证实了分类依赖的表型转变，由于相互作用。总之，我们的数据表明，细菌的相互作用可能是混合微生物种群的表型异质性的一般驱动因素。重要实验室研究表明，表型异质性对同基因种群的生存和功能的影响。由于表型异质性在致病性和毒力，抗生素抗性，生物技术应用和生态系统特性中起着重要作用，因此了解其影响因素至关重要。一个尚未回答的问题是，混合群落中的细菌是否会影响其群落伙伴的表型异质性。我们发现，共培养的细菌导致其个人的表型异质性的减少，这使我们的假设，即一个分类单元的个人表型多样性是依赖于社会组成。
Isogenic bacterial populations are known to exhibit phenotypic heterogeneity at the single-cell level. Because of difficulties in assessing the phenotypic heterogeneity of a single taxon in a mixed community, the importance of this deeper level of organization remains relatively unknown for natural communities. In this study, we have used membrane-based microcosms that allow the probing of the phenotypic heterogeneity of a single taxon while interacting with a synthetic or natural community. Individual taxa were studied under axenic conditions, as members of a coculture with physical separation, and as a mixed culture. Phenotypic heterogeneity was assessed through both flow cytometry and Raman spectroscopy. Using this setup, we investigated the effect of microbial interactions on the individual phenotypic heterogeneities of two interacting drinking water isolates. Through flow cytometry we have demonstrated that interactions between these bacteria lead to a reduction of their individual phenotypic diversities and that this adjustment is conditional on the bacterial taxon. Single-cell Raman spectroscopy confirmed a taxon-dependent phenotypic shift due to the interaction. In conclusion, our data suggest that bacterial interactions may be a general driver of phenotypic heterogeneity in mixed microbial populations.IMPORTANCE Laboratory studies have shown the impact of phenotypic heterogeneity on the survival and functionality of isogenic populations. Because phenotypic heterogeneity plays an important role in pathogenicity and virulence, antibiotic resistance, biotechnological applications, and ecosystem properties, it is crucial to understand its influencing factors. An unanswered question is whether bacteria in mixed communities influence the phenotypic heterogeneity of their community partners. We found that coculturing bacteria leads to a reduction in their individual phenotypic heterogeneities, which led us to the hypothesis that the individual phenotypic diversity of a taxon is dependent on the community composition.