Polygonum hydropiper extract attenuates ethanol-induced gastric damage through antioxidant and anti-inflammatory pathways.
Polygonum hydropiper extract attenuates ethanol-induced gastric damage through antioxidant and anti-inflammatory pathways.
复制标题
蓼提取物通过抗氧化和抗炎途径减轻乙醇引起的胃损伤
DOI：
10.1590/1414-431x2020e10841
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发表时间：
2021
期刊：
影响因子：
--
通讯作者：
Xie Y
中科院分区：
文献类型：
--
作者：
Ren S;Chen B;Ma Z;Hu H;Xie Y
本研究旨在探讨辣蓼抗乙醇性急性胃粘膜损伤的作用机制及有效成分。乙醇提取物经AB-8大孔吸附树脂柱纯化后，用60%乙醇洗脱，进样HPLC进行定量分析。Sprague-Dawley大鼠经口预处理与P. hydropiper提取物（PHLE; 50，100，和200 mg/kg）5天，然后给予无水乙醇诱导胃粘膜损伤。摄入乙醇后1小时，对大鼠实施安乐死，并收集胃样品进行生化分析。抗氧化酶和抗炎细胞因子进行定量。Western blotting检测蛋白表达水平。CCK-8法检测细胞增殖。水辣蓼提取物中总黄酮含量为50.05%，含有芦丁、槲皮苷和槲皮素3种主要的黄酮类活性成分。PHLE能显著提高细胞活力，并有效地保护人胃上皮细胞-1免受酒精诱导的损伤。PHLE预处理以剂量依赖方式减轻大鼠胃粘膜损伤，并增加胃组织中超氧化物歧化酶、谷胱甘肽过氧化物酶和谷胱甘肽的活性，降低丙二醛的水平。PHLE预处理还通过下调核因子-κ B的表达减少胃组织中促炎细胞因子肿瘤坏死因子-α和白细胞介素-1 β的产生。PHLE通过抗氧化和抗炎途径对胃损伤具有保护作用。黄酮类化合物可能是水辣蓼抗胃粘膜损伤的主要有效成分。
The present study was conducted to investigate the underlying mechanisms and effective components of Polygonum hydropiper in ethanol-induced acute gastric mucosal lesions. The ethanol extract was purified on an AB-8 macroporous resin column and eluted with 60% ethanol and was then injected into the HPLC system for quantitative analysis. Sprague-Dawley rats were orally pretreated with P. hydropiper extract (PHLE; 50, 100, and 200 mg/kg) for 5 days and then absolute ethanol was administered to induce gastric mucosal damage. One hour after ethanol ingestion, the rats were euthanized and stomach samples were collected for biochemical analysis. Antioxidant enzymes and anti-inflammatory cytokines were quantified. Western blotting was used to detect the expression levels of proteins. Cell proliferation was assayed by CCK-8 assays. The proportion of total flavonoids in the final extract of P. hydropiper was 50.05%, which contained three major bioactive flavonoid constituents, including rutin, quercitrin, and quercetin. PHLE significantly increased cell viability and effectively protected human gastric epithelial cells-1 against alcohol-induced damage in vitro. PHLE pretreatment attenuated gastric mucosal injuries in a dose-dependent manner in rats, and increased the activity of superoxide dismutase, glutathione peroxidase, and glutathione, and decreased the levels of malondialdehyde in gastric tissue. Pretreatment with PHLE also reduced the generation of the pro-inflammatory cytokines tumor necrosis factor-α and interleukin-1β in gastric tissue by downregulating the expression of nuclear factor-kappa B. PHLE exerted protective effects against gastric injury through antioxidant and anti-inflammatory pathways. Flavonoids might be the main effective components of P. hydropiper against gastric mucosal injury.