Characterization of the minimal replicator of Kaposi's sarcoma-associated herpesvirus latent origin
Characterization of the minimal replicator of Kaposi's sarcoma-associated herpesvirus latent origin
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DOI：
10.1128/jvi.79.4.2637-2642.2005
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发表时间：
2005-02-01
影响因子：
5.4
通讯作者：
Renne, R
中科院分区：
文献类型：
--
作者：
Hu, JH;Renne, R
卡波西氏肉瘤相关疱疹病毒（KSHV）的潜伏期相关核抗原（LANA）结合到801 bp长的末端重复序列（TR）中的两个位点上，并且是维持表观所需的唯一病毒蛋白。虽然长期维持需要两个或更多的TR拷贝，但单个TR赋予质粒DNA上依赖lana的起源活性。缺失图谱显示一个71 bp长的最小复制子包含两个不同的序列元素：LANA结合位点（LBS1/2）和一个相邻的29- 32 bp长的富含gc的序列，我们称之为复制元素。此外，转录因子Sp1可以与最小复制子外的TR结合，并有助于TR先前报道的增强子活性。
The latency-associated nuclear antigen (LANA) of Kaposi's sarcoma-associated herpesvirus (KSHV) binds to two sites within the 801-bp-long terminal repeat (TR) and is the only viral protein required for episomal maintenance. While two or more copies of TR are required for long-term maintenance, a single TR confers LANA-dependent origin activity on plasmid DNA. Deletion mapping revealed a 71-bp-long minimal replicator containing two distinctive sequence elements: LANA binding sites (LBS1/2) and an adjacent 29- to 32-bp-long GC-rich sequence which we termed the replication element. Furthermore, the transcription factor Sp1 can bind to TR outside the minimal replicator and contributes to TR's previously reported enhancer activity.