Change in late sodium current of atrial myocytes in spontaneously hypertensive rats with allocryptopine treatment
Change in late sodium current of atrial myocytes in spontaneously hypertensive rats with allocryptopine treatment
复制标题
别隐品治疗自发性高血压大鼠心房肌细胞晚钠电流的变化
DOI：
10.5830/cvja-2018-072
复制
发表时间：
2019-03-01
影响因子：
0.7
通讯作者：
Li, Yang
中科院分区：
文献类型：
--
作者：
Dong, Ying;Huang, Yun;Li, Yang
目的：目的：研究别隐品碱（allocyptopine，All）对自发性高血压大鼠（SHR）心房肌细胞晚钠电流（I-Na，I-Late）的影响。结果：与WKY大鼠细胞相比，SHR心肌细胞I-Na，I-Late电流明显增加，30 μ M的Al 3处理后，电流密度明显降低，I-Na，I-Late/I-Na，I-peak比值降低。所有降低I-Na，I-Late通过减轻通道的失活和增加钠通道的窗口电流。此外，I-Na，I-Late密度的三个SCN 5A突变与30 μ M的所有浓度依赖manner.Conclusion：结果清楚地表明，增加的I-Na，I-Late在SHR心房肌细胞抑制由所有来自中国草药。
Aim: We aimed to study the effect of allocryptopine (All) on the late sodium current (I-Na,I-Late) of atrial myocytes in spontaneously hypertensive rats (SHR).Methods: The enzyme digestion method was used to separate single atrial myocytes from SHR and Wistar-Kyoto (WKY) rats. I-Na,I-Late was recorded using the patch-clamp technique, and the effect of All was evaluated on the current.Results: Compared with WKY rat cells, an increase in the I-Na,I-Late current in SHR myocytes was found. After treatment with 30 mu M All, the current densities were markedly decreased; the ratio of I-Na,I-Late/I-Na,I-peak of SHR was reduced by30 mu M All. All reduced I-Na,I-Late by alleviating inactivation of the channel and increasing the window current of the sodium channel. Furthermore, I-Na,I-Late densities of three SCN5A mutations declined substantially with 30 mu M All in a concentration-dependent manner.Conclusion: The results clearly show that an increase in I-Na,I-Late in SHR atrial myocytes was inhibited by All derived from Chinese herbal medicine.