Synthesis of the second component of complement by long-term primary cultures of human monocytes
Synthesis of the second component of complement by long-term primary cultures of human monocytes
复制标题
人单核细胞长期原代培养物合成补体第二成分
DOI：
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发表时间：
1976
影响因子：
15.3
通讯作者：
N. H. R. Colten
中科院分区：
文献类型：
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作者：
B. L. P. Einstein;Eveline E. Schneeberger;N. H. R. Colten
已经开发了一种方法，用于从少量血液中制备人单核细胞的汇合单层，并用于在体外维持这些纯单核细胞培养物长达16周。这些细胞吞噬5.7 μ m直径的乳胶珠，玫瑰红细胞与IgG或C3包被，杀死李斯特菌，并合成溶菌酶和补体的第二组分。在培养物中，溶菌酶以约50，000 mol/min/细胞的速率分泌至少12周。C2合成和分泌的最大速率相当低;即，在培养的第2和第12周之间，每个细胞约30 mol/min。单核细胞在培养的前6天内几乎不产生C2，之后注意到C2产生速率显著增加。这种增加与单核细胞成熟的形态学证据一致。
A method has been developed for preparation of confluent monolayers of human monocytes from small volumes of blood and for maintenance of these pure monocyte cultures for up to 16 wk in vitro. These cells phagocytosed 5.7 mum diameter latex beads, rosetted with erythrocytes coated with IgG or with C3, killed Listeria monocytogenes, and synthesized both lysozyme and the second component of complement. Lysozyme was secreted at a rate of approximately 50,000 mol/min per cell for at least 12 wk in cultures. The maximal rate of C2 synthesis and secretion was considerably less; i.e., approximately 30 mol/min per cell between the 2nd and 12th wk in culture. Monocytes produced little C2 during the first 6 days in culture after which a marked increase in the rate of C2 production was noted. This increase was coincident with morphologic evidence of monocyte maturation.