Relationship between pine wilt-tolerance rankings of Pinus thunbergii trees and the number of Bursaphelenchus xylophilus passing through branch sections
Relationship between pine wilt-tolerance rankings of Pinus thunbergii trees and the number of Bursaphelenchus xylophilus passing through branch sections
复制标题
黑松耐枯萎病等级与松材线虫穿过枝节数量的关系
DOI：
10.1163/15685411-00003108
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发表时间：
2017
期刊：
影响因子：
1.2
通讯作者：
K. Matsunaga and K. Togashi
中科院分区：
文献类型：
--
作者：
Menendez-Gutierrez;M.;K. Matsunaga and K. Togashi
为了建立一种简单、省时的方法来筛选耐松材线虫病的松树候选菌株，进行了3个试验。当两个致病菌株SC-9和T-4接种在枝条切端时，能抑制更多的线虫通过枝条，而不是枝条。SC-9倾向于通过P。雷击比T-4更容易。当接种线虫200条时，线虫通过5厘米长的松枝段的概率较大。将200条SC-9线虫接种于5 cm、10 cm或15 cm长的枝条上。8月下旬不同耐性等级的松材线虫无性系，耐性等级OFP与通过5 cm长枝段的线虫数量相关性不显著，但在8月中旬，线虫扩散能力可以区分耐性和感病的松树无性系，表明这是一种有助于选择耐性树种的方法。耐受性水平的季节性突然上升表明，高温和干旱等天气条件可能扰乱了抗病物种OFP的耐受机制。
To establish a simple, time-saving method for selectingPinustree candidates tolerant to the infection ofBursaphelenchus xylophilus, the causative agent of pine wilt disease, three experiments were conducted. When two virulent isolates Sc-9 and T-4 were inoculated on cut ends of branch sections, more nematodes were inhibited from passing throughP. taedabranch sections thanP. thunbergiisections. Sc-9 tended to pass throughP. thunbergiisections more easily than T-4. The probability of nematodes passing through 5-cm-longP. thunbergiibranch sections was greater when 200 nematodes were inoculated. When 200 Sc-9 nematodes were inoculated on 5-, 10- or 15-cm-long branch sections ofP. taedatrees andP. thunbergiiclones of different tolerance classes in late August, an insignificant correlation was found between the tolerance class ofP. thunbergiiclones and the number of nematodes passing through 5-cm-long branch sections, although nematode dispersal ability could distinguish between tolerant and susceptible pine clones in mid-August, suggesting it was a helpful method for selecting tolerant tree candidates. Abrupt seasonal increases in tolerance level suggested that weather conditions, such as hot temperatures and drought, may have disrupted the tolerance mechanism of resistant species ofP. taeda.