CHARACTERIZATION OF ANTI-REOVIRUS IMMUNOGLOBULINS SECRETED BY CLONED HYBRIDOMA CELL-LINES
CHARACTERIZATION OF ANTI-REOVIRUS IMMUNOGLOBULINS SECRETED BY CLONED HYBRIDOMA CELL-LINES
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DOI：
10.1016/0042-6822(81)90533-x
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发表时间：
1981-01-01
期刊：
影响因子：
3.7
通讯作者：
JOKLIK, WK
中科院分区：
文献类型：
--
作者：
LEE, PWK;HAYES, EC;JOKLIK, WK
克隆了 19 个源自用呼肠孤病毒血清型 3（Dearing 株）免疫的小鼠脾细胞的杂交瘤细胞系，并对它们分泌的 IgG 进行了表征。四种IgG针对多肽.mu.NS，两种IgG针对多肽.sigma.NS，这两种非结构性呼肠孤病毒特异性多肽。两个IgG针对多肽λ2，2个IgG针对多肽σ2，两者都是呼肠孤病毒衣壳内壳的组成部分。 4个IgG针对多肽σ1，所有呼肠孤病毒特异性多肽中最具类型特异性的，3个IgG针对多肽.mu.1/.mu.1C，1个IgG针对多肽σ3，即呼肠孤病毒外壳的所有成分。一种IgG针对由多肽.mu.1/.mu.1C和σ3组成的复合物，但未能与任一多肽单独反应。因此，19 个杂交瘤细胞系分泌的 IgG 对 10 种呼肠孤病毒特异性蛋白中的 7 种具有特异性。测量了所有这些 IgG 沉淀 2 种异源呼肠孤病毒血清型（血清型 1 和 2）特异蛋白质的能力。
Nineteen hybridoma cell lines derived from the spleen cells of a mouse immunized with reovirus serotype 3 (strain Dearing) were cloned and the IgG secreted by them were characterized. Four IgG were directed against polypeptide .mu.NS and 2 against polypeptide .sigma.NS, the 2 nonstructural reovirus-specified polypeptides. Two IgG were directed against polypeptide .lambda.2 and 2 against polypeptide .sigma.2, both components of the reovirus inner capsid shell. Four IgG were directed against polypeptide .sigma.1, the most type specific of all reovirus-specified polypeptides, 3 against polypeptide .mu.l/.mu.lC and 1 against polypeptide .sigma.3, all components of the reovirus outer capsid shell. One IgG was directed against a complex composed of polypeptides .mu.l/.mu.lC and .sigma.3, but failed to react with either polypeptide alone. Thus the 19 hybridoma cell lines secreted IgG with specificities for 7 of the 10 reovirus-specified proteins. The ability of all these IgG to precipitate the proteins specified by the 2 heterologous reovirus serotypes, serotypes 1 and 2, was measured.