Strigolactones regulate protonema branching and act as a quorum sensing-like signal in the moss Physcomitrella patens
Strigolactones regulate protonema branching and act as a quorum sensing-like signal in the moss Physcomitrella patens
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DOI：
10.1242/dev.058495
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发表时间：
2011-04-15
期刊：
影响因子：
4.6
通讯作者：
Rameau, Catherine
中科院分区：
文献类型：
--
作者：
Proust, Helene;Hoffmann, Beate;Rameau, Catherine
独角孤内酯是一类控制种子植物茎枝分枝的新型植物激素。在共生和寄生相互作用中，它们也表明寄主根系接近。为了更好地了解独脚金内酯功能的起源，我们对一个苔藓突变体进行了表征，该突变体在类胡萝卜素裂解双加氧酶8 （CCD8）基因缺失后，强烈影响了独脚金内酯的生物合成。在本研究中，我们发现野生型小立小藻在培养基中产生并释放独角酯内酯，并控制原丝的分支和菌落的扩展。我们进一步表明，Ppccd8突变菌落不能感知邻近菌落的接近，这在野生型植物中导致菌落扩展受阻。当在野生型菌落附近生长时，通过外源人工合成的独角独内酯或种子植物CCD8的异位表达，突变体表型得以恢复。因此，我们的数据首次证明苔藓植物（P. patens）产生的独角麦内酯作为信号因子控制发育和潜在的生态生理过程。我们提出，在P. patens中，独角酯内酯让人想起细菌用来相互交流的群体感应分子。
Strigolactones are a novel class of plant hormones controlling shoot branching in seed plants. They also signal host root proximity during symbiotic and parasitic interactions. To gain a better understanding of the origin of strigolactone functions, we characterised a moss mutant strongly affected in strigolactone biosynthesis following deletion of the CAROTENOID CLEAVAGE DIOXYGENASE 8 (CCD8) gene. Here, we show that wild-type Physcomitrella patens produces and releases strigolactones into the medium where they control branching of protonemal filaments and colony extension. We further show that Ppccd8 mutant colonies fail to sense the proximity of neighbouring colonies, which in wild-type plants causes the arrest of colony extension. The mutant phenotype is rescued when grown in the proximity of wild-type colonies, by exogenous supply of synthetic strigolactones or by ectopic expression of seed plant CCD8. Thus, our data demonstrate for the first time that Bryophytes (P. patens) produce strigolactones that act as signalling factors controlling developmental and potentially ecophysiological processes. We propose that in P. patens, strigolactones are reminiscent of quorum-sensing molecules used by bacteria to communicate with one another.