Activation of synaptic NMDA receptors induces membrane insertion of new AMPA receptors and LTP in cultured hippocampal neurons
Activation of synaptic NMDA receptors induces membrane insertion of new AMPA receptors and LTP in cultured hippocampal neurons
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DOI：
10.1016/s0896-6273(01)00194-5
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发表时间：
2001-01-01
期刊：
影响因子：
16.2
通讯作者：
Wang, YT
中科院分区：
文献类型：
--
作者：
Lu, WY;Man, HY;Wang, YT
海马兴奋性传递的长时程增强（LTP）可能有助于学习和记忆。尽管AMPA受体的磷酸化和再分布可能是这种突触可塑性形式的原因，但这些突触中LTP的潜在机制尚未完全清楚。我们在此表明，当用甘氨酸短暂刺激突触后NMDA受体时，培养的海马神经元中的微小兴奋性突触后电流（mEPSCs）可靠地显示出LTP。这些突触的LTP伴随着天然AMPA受体的快速插入以及AMPA受体在树突膜表面聚集的增加。LTP和甘氨酸促进的AMPA受体插入均被细胞内破伤风毒素（TeTx）阻断，这提供了证据表明在LTP期间AMPA受体通过一种依赖SNARE的胞吐作用插入兴奋性突触。
Long-term potentiation (LTP) of excitatory transmission in the hippocampus likely contributes to learning and memory. The mechanisms underlying LTP at these synapses are not well understood, although phosphorylation and redistribution of AMPA receptors may be responsible for this form of synaptic plasticity. We show here that miniature excitatory postsynaptic currents (mEPSCs) in cultured hippocampal neurons reliably demonstrate LTP when postsynaptic NMDA receptors are briefly stimulated with glycine. LTP of these synapses is accompanied by a rapid insertion of native AMPA receptors and by increased clustering of AMPA receptors at the surface of dendritic membranes. Both LTP and glycine-facilitated AMPA receptor insertion are blocked by intracellular tetanus toxin (TeTx), providing evidence that AMPA receptors are inserted into excitatory synapses via a SNARE-dependent exocytosis during LTP.